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CnT-17

  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-17 Airway Epithelial Proliferation Medium

Efficient isolation and expansion of large airway epithelial cells.

Catalog CnT-17
Content 500 ml basal medium + supplement pack

HIGHLIGHTS:

  • Market leading precision medium for isolation and expansion of large airway epithelial cells
  • Chemically defined

Description

CnT-17 is a fully defined medium for isolation and expansion of large airway epithelial cells.

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Specifications

Tissue type
Airway epithelial cells
Application
Isolation / Expansion
Serum
No
BPE
No
Free of Human & Animal Component
No
Chemically defined
Yes
Clinically upgradable
Yes
Volume
500 ml
Component(s)
Basal Medium & Supplement Pack
Quality level
Research Grade

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Scientific resources

CnT-17 is a low calcium medium formulation designed for isolation and expansion of mouse and human epithelial cells. Is the original formulation from which the CnT-PR-A medium was developed. It is provided as a liquid culture medium kit including both basal medium and supplements.

For maximum isolation efficiency from epithelial tissues, it is recommended to supplement CnT-17 with the IsoBoost supplement (#CnT-ISO-50), which has been found to double cell yield in the first week of culture.

Primary epithelial cells growing in CnT-17 display a highly proliferative phenotype. For differentiation experiments, it is recommended to switch to the CnT-PR-AD differentiation medium. Please see Protocols Page for more information.

The medium undergoes a range of QC tests in our lab before being released for sale. Please see the datasheet for details. It can easily be upgraded for clinical applications. Contact us at support@cellntec.com for further details and pricing.

Like to try a test sample? Click here for more information.

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Scientific Literature

Title Authors Year Tissue type
Ferret airway epithelial cell cultures support efficient replication of influenza B virus but not mumps virus Ruth A. Elderfield, Lauren Parker, Peter Stilwell, Kim L. Roberts, Silke Schepelmann, Wendy S. Barclay 2015 Airway
Imaging viscosity of intragranular mucin matrix in cystic fibrosis cells Sebastian Requena, Olga Ponomarchuk, Marlius Castillo, Jonathan Rebik, Emmanuelle Brochiero, Julian Borejdo, Ignacy Gryczynski, Sergei V. Dzyuba, Zygmunt Gryczynski, Ryszard Grygorczyk & Rafal Fudala 2017 Airway
SARS-CoV-2 Spike Expression at the Surface of Infected Primary Human Airway Epithelial Cells Shilei Ding, Damien Adam, Guillaume Beaudoin-Bussières, Alexandra Tauzin, Shang Yu Gong, Romain Gasser, Annemarie Laumaea, Sai Priya Anand, Anik Privé, Catherine Bourassa, Halima Medjahed, Jérémie Prévost, Hugues Charest, Jonathan Richard, Emmanuelle Brochiero , and Andrés Finzi 2021 Airway

For more publications on CnT-17 from our literature database, see here

Title Year
CELLnTEC Catalog 2023 Download

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