CELLnTEC / Products / HPEKs


CnT Epidermal Keratinocyte Progenitors, Juvenile, Single Donor

Normal human epidermal keratinocyte progenitor cells, isolated from juvenile donor material, cryo-preserved at early passage.

Catalog HPEKs
Content Vial containing 0.5 million viable cells, frozen


  • High-quality cells
  • Pre-equilibrated in CELLnTEC base chemistry
  • Supplied in fully-defined cryopreservation medium to maximize recovery after thawing


HPEKs primary human epidermal keratinocytes (single donor) growing in CnT-PR medium benefit from the presence of PCT factors that aid retention of proliferative progenitors and reduce differentiation. HANDLING INSTRUCTION: This material is of human origin and should therefore be handled as hazardous. Although it has been tested negative for HIV 1, Hepatitis B, and Hepatitis C, treat this material as potentially infectious and use appropriate biocontainment, protective equipment, and other precautions to prevent accidental exposure.

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Primary Cells
Tissue type
Cell type
Epidermal Keratinocyte Progenitors
Pack size
0.5 million viable cells (1 mL), frozen vial
Culture Medium
CnT-07 / CnT-57 / CnT-PR

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Scientific resources

The culturing medium CnT-PR consists of PCT factors that aid the retention of proliferative progenitors and reduce differentiation of HPEKs cells. This extends not only the lifespan of the cells but importantly also the period during which the cells retain the ability to complete complex processes such as 3D differentiation.

The HPEK cells are provided cryopreserved in the fully defined CRYO-Defined culture medium to maximize recovery after thawing.

The cultures undergo a range of QC tests in our lab before being released for sale and are also covered by our longevity guarantee. Please see the datasheet for details.

The thawing, seeding and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.

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Scientific Literature

Title Authors Year Tissue type
Beneficial Effects of the Genus Aloe on Wound Healing, Cell Proliferation, and Differentiation of Epidermal Keratinocytes Mariko Moriyama, Hiroyuki Moriyama, Junki Uda, Hirokazu Kubo, Yuka Nakajima, Arisa Goto, Junji Akaki, Ikuyo Yoshida, Nobuya Matsuoka, Takao Hayakawa 2016 Epidermal
In vitro skin culture media influence the viability and inflammatory response of primary macrophages Chiara Griffoni, Berna Neidhart, Ke Yang, Florian Groeber-Becker, Katharina Maniura-Weber, Thomas Dandekar, Heike Walles, Markus Rottmar 2021 Epidermal
Partially Hydrolysed Whey-Based Infant Formula Improves Skin Barrier Function Sébastien Holvoet, Sophie Nutten, Lénaïck Dupuis, Dominique Donnicola, Tristan Bourdeau, Betsy Hughes-Formella, Dagmar Simon, Hans-Uwe Simon, Ryan S. Carvalho, Jonathan M. Spergel, Sibylle Koletzko, Carine Blanchard 2021 Epidermal

For more publications on HPEK from our literature database, see here

Title Year
CELLnTEC Catalog 2023 Download

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