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CnT-ISO-50-HC

  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-IsoBoost, Isolation Boosting Supplement, Higher Certified

A chemically defined and clinically ready supplement added to the culture medium after isolation, that significantly improves viability and attachment of primary epithelial cells.

Catalog CnT-ISO-50-HC
Content 50 uL of 1000x concentrate, frozen

HIGHLIGHTS:

  • Chemically defined, contains no components of animal or human origin
  • Significantly increases isolation efficiency
  • Extended QC, documentation, support, and process control for clinical researchers

Description

A sterile, 1000 x ready-to-use concentrate which is added to the culture medium for the first 3 days of culture immediately after isolation. Significantly improves viability and attachment of primary human cells.

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Specifications

Tissue type
Epithelial cells
Species
Human & Mouse
Application
Isolation
Free of Human & Animal Component
Yes
Chemically defined
Yes
Clinically upgradable
No
Volume
50 ul
Quality level
Higher Certified

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Scientific resources

CnT-IsoBoost Supplement

Addition of the CnT-IsoBoost supplement to CnT-PR medium for the first 3 days after isolation more than doubles the isolation efficiency.

Isolation is a considerable stress for primary cells, in which the physical detachment and loss of cohesion results in significant loss of viability and reduced attachment.

The new Higher Certified version of IsoBoost supplement (CnT-ISO-50-HC) is able to significantly boost the isolation efficiency of primary human epithelial cells by protecting them against stress and improving viability and attachment rates.

In tests with the CnT-PR medium (left), more than twice as many population doublings were achieved in the first 7 days of culture when the CnT-ISO-50 supplement was added during the first 3 days of culture. This resulted in 5x more cells than in standard medium alone:

IsoBoost delivers 5x more cells on day 7 post isolation.

CnT-ISO-50_HPEK_d7
CnT-ISO-50 delivers 5x more cells on day 7 post isolation.

Figure: Primary human keratinocytes (HPEK), day 7 after isolation in CnT-PR plus IsoBoost supplement.

Low cost test samples are available now!

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Scientific Literature

Title Year
CELLnTEC Catalog 2020 Download

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