- Maintain structure and function as opposed to rapid growth
- Fully supplemented, one bottle ready-to-go
- Chemically defined, contains no components of animal or human origin
Specifically designed for tissue homeostasis and enables the maintenance of tissue biopsies in explant culture for more than 7 days without loss of viability or tissue structure (for explant culture and transportation).
|Content||250 ml, Frozen medium|
CnT-XP3 Biopsy Maintenance Medium is a chemically defined and animal component-free medium specifically designed for tissue biopsy maintenance. It is ready to use as provided.
Traditional culture media are designed to deliver maximum proliferation rates of cells in culture. They are not optimal for maintaining tissue biopsies during transport or in the period prior to cell isolation.The CnT-XP3 Biopsy Maintenance Medium is specifically designed for tissue homeostasis and enables the maintenance of tissue biopsies in explant culture for more than 7 days without loss of viability or tissue structure.
It is fully defined, free of all human and animal-derived components, and is thus well suited for use in clinical applications. Biopsies maintained in XP3 for up to 3 days still yield high quality RNA (RIN above 8.0), and 25% higher cell yield after isolation.
Figure: Keratinocyte yield from biopsies maintained in CnT-XP3 medium is 25% higher than control biopsies maintained in regular culture medium.
Thus when combining the increased initial cell yield with the faster growth in early culture, the use of CnT-XP3 increased cell yield x5 at the end of week 1, in comparison with the use of the standard medium for biopsy maintenance.
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|Thermal conditioning improves quality and speed of keratinocyte sheet production for burn wound treatment||Laura Frese, Salim E. Darwiche, Brigitte von Rechenberg, Simon P. Hoerstrup, Pietro Giovanoli, Maurizio Calcagni||2021||Epidermal|