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CnT-XP3

  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-XP3 Biopsy Maintenance Medium

Specifically designed for tissue homeostasis and enables the maintenance of tissue biopsies in explant culture for more than 7 days without loss of viability or tissue structure (for explant culture and transportation).

Catalog CnT-XP3
Content 250 ml, Frozen medium

HIGHLIGHTS:

  • Maintain structure and function as opposed to rapid growth
  • Fully supplemented, one bottle ready-to-go
  • Chemically defined, contains no components of animal or human origin

Description

CnT-XP3 Biopsy Maintenance Medium is a chemically defined and animal component-free medium specifically designed for tissue biopsy maintenance. It is ready to use as provided.

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Specifications

Application
Isolation
Free of Human & Animal Component
Yes
Chemically defined
Yes
Clinically upgradable
No
Volume
250 ml
Quality level
Research Grade

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Scientific resources

xp3_comparison

Skin biopsy structure is maintained much better after 10 days in CnT-XP3 (A), compared with competitor media (B & C). Click for full image.

Traditional culture media are designed to deliver maximum proliferation rates of cells in culture. They are not optimal for maintaining tissue biopsies during transport or in the period prior to cell isolation.

The CnT-XP3 Biopsy Maintenance Medium is specifically designed for tissue homeostasis and enables the maintenance of tissue biopsies in explant culture for more than 7 days without loss of viability or tissue structure.

It is fully defined, free of all human and animal-derived components, and is thus well suited for use in clinical applications. Biopsies maintained in XP3 for up to 3 days still yield high quality RNA (RIN above 8.0), and 25% higher cell yield after isolation.
 
 
xp3_isolation_web
Figure: Keratinocyte yield from biopsies maintained in CnT-XP3 medium is 25% higher than control biopsies maintained in regular culture medium.

Cells isolated from tissue biopsies maintained in CnT-XP3 prior to cell isolation also demonstrate higher functionality and deliver much stronger proliferation during their first week in culture.
After 7 days of growth in CnT-PR medium (initial seeding 4’000 cells/cm2), primary human keratinocyte cultures established from tissue biopsies maintained in CnT-XP3 prior to isolation were found to have 4x more cells than cultures established from control biospies maintained in standard culture medium prior to isolation.
 
 
xp3_expansion_web
Figure: Keratinocytes isolated from biopsies maintained in XP3 deliver 4x more cells at the end of the first passage of expansion.

 
Thus when combining the increased initial cell yield with the faster growth in early culture, the use of CnT-XP3 increased cell yield x5 at the end of week 1, in comparison with the use of the standard medium for biopsy maintenance.

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Scientific Literature

Title Year
CELLnTEC Catalog 2020 Download

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