CELLnTEC / Products / CnT-07


  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-07 Epithelial Proliferation Medium

Efficient isolation and expansion of epithelial cells from multiple tissue types

Catalog CnT-07
Content 500 ml basal medium + supplement pack


  • Market leading precision medium for isolation and expansion of epithelial cells
  • Chemically defined


CnT-07 is a fully defined medium for mouse and human keratinocyte culture.

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Tissue type
Epithelial cells
Human & Mouse
Isolation / Expansion
Free of Human & Animal Component
Chemically defined
Clinically upgradable
500 ml
Basal Medium & Supplement Pack
Quality level
Research Grade

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Scientific resources

CnT-07 is a low calcium medium formulation designed for isolation and expansion of mouse and human epithelial cells. Is the original formulation from which the CnT-PR medium was developed. It is provided as a liquid culture medium kit including both basal medium and supplements.

For maximum isolation efficiency from epithelial tissues, it is recommended to supplement CnT-07 with the IsoBoost supplement (#CnT-ISO-50), which has been found to double cell yield in the first week of culture.

Primary epithelial cells growing in CnT-07 display a highly proliferative phenotype. For differentiation experiments, it is recommended to switch to the CnT-PR-D or CnT-PR-3D differentiation media.

The medium undergoes a range of QC tests in our lab before being released for sale. Please see the datasheet for details. It is available in an upgraded Higher Certified version for clinical applications. Please visit CnT-07-HC product page for further details.

Like to try a test sample? Click here for more information.

Thawing, seeding and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.


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Scientific Literature

Title Authors Year Tissue type
Expression of Desmoglein 2, Desmocollin 3 and Plakophilin 2 in Placenta and Bone Marrow-Derived Mesenchymal Stromal Cells Melanie L. Hart & Elisa Rusch & Marvin Kaupp & Kay Nieselt & Wilhelm K. Aicher 2017 Bladder
Isolation and Cultivation of Epidermal (Stem) Cells Xusheng Wang, Shiyang Dong, Yaojiong Wu 2018 Epidermal
Laminin-332 regulates differentiation of human interfollicular epidermal stem cells Takaaki Yamada, Seiji Hasegawa, Katsuma Miyachi, Yasushi Date, Yu Inoue, Akiko Yagami, Masaru Arima, Yohei Iwata, Naoki Yamamoto, Satoru Nakata, Kayoko Matsunaga, Kazumitsu Sugiura, Hirohiko Akamatsu 2018 Epidermal

For more publications on CnT-07 from our literature database, see here

Title Year
CELLnTEC Catalog 2020 Download

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