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CnT-07

  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-07 Epithelial Proliferation Medium

Isolation and expansion of epithelial cells from multiple tissue types. Chemically defined.

Catalog CnT-07
Content 500 ml basal medium + supplement pack

HIGHLIGHTS:

  • Isolation and expansion of epithelial cell types including primary keratinocytes
  • Chemically defined

Description

CnT-07 is a fully defined medium for isolation and expansion of a broad range epithelial cell types including primary keratinocytes. No feeder cells or plate coating are required.

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Specifications

Tissue type
Epithelial cells & Keratinocyte
Species
Human and mouse, may also work for other species
Application
Isolation / Expansion
Serum-Free
Yes
BPE-Free
Yes
Free of Human & Animal Component
No
Chemically defined
Yes
Clinically upgradable
Yes
Volume
500 ml
Component(s)
Basal Medium & Supplement Pack
Quality level
Research Grade

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Scientific resources

CnT-07 is a low calcium medium formulation designed for isolation and expansion of mouse and human epithelial cells. Is the original formulation from which the CnT-PR medium was developed. It is provided as a liquid culture medium kit including both basal medium and supplements.

For maximum isolation efficiency from epithelial tissues, it is recommended to supplement CnT-07 with the IsoBoost supplement (#CnT-ISO-50), which has been found to double cell yield in the first week of culture.

Primary epithelial cells growing in CnT-07 display a highly proliferative phenotype. For differentiation experiments, it is recommended to switch to the CnT-PR-D or CnT-PR-3D differentiation media.

The medium undergoes a range of QC tests in our lab before being released for sale. Please see the datasheet for details. It is available in an upgraded Higher Certified version for clinical applications. Please visit CnT-07-HC product page for further details.

Like to try a test sample? Click here for more information.

Thawing, seeding and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.

 

PRODUCT SELECTION TABLE


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Scientific Literature

Title Authors Year Tissue type
Characterization of Rickets Type II Model Rats to Reveal Functions of Vitamin D and Vitamin D Receptor Yuichiro Iwai, Ayano Iijima, Satoko Kise, Chika Nagao, Yuto Senda, Kana Yabu, Hiroki Mano, Miyu Nishikawa, Shinichi Ikushiro, Kaori Yasuda, Toshiyuki Sakaki, 2023 Epidermal
Enhancer hijacking activates oncogenic transcription factor NR4A3 in acinic cell carcinomas of the salivary glands Florian Haller, Matthias Bieg, Rainer Will, Cindy Körner, Dieter Weichenhan, Alexander Bott, Naveed Ishaque, Pavlo Lutsik, Evgeny A. Moskalev, Sarina K. Mueller, Marion Bähr, Angelika Woerner, Birgit Kaiser, Claudia Scherl, Marlen Haderlein, Kortine Kleinheinz, Rainer Fietkau, Heinrich Iro, Roland Eils, Arndt Hartmann, Christoph Plass, Stefan Wiemann, Abbas Agaimy 2019 Other
Expression of Desmoglein 2, Desmocollin 3 and Plakophilin 2 in Placenta and Bone Marrow-Derived Mesenchymal Stromal Cells Melanie L. Hart & Elisa Rusch & Marvin Kaupp & Kay Nieselt & Wilhelm K. Aicher 2017 Bladder

For more publications on CnT-07 from our literature database, see here

Title Year
CELLnTEC Catalog 2023 Download

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