High-efficiency isolation and expansion of fibroblasts in a serum-free, clinically ready formulation and without requiring any plate coating.
| Catalog | CnT-PR-F-HC |
|---|---|
| Content | 500 ml, Frozen medium |
CnT-PR-F-HC medium is a serum-free formulation that delivers improved isolation efficiency and proliferation of human and animal fibroblasts. It is ideally suited for use during product development, protocol validation, and characterization of the cell-based product.
CnT-PR-F delivers 50% more cells on day 5 post isolation compared with traditional 10% serum media.
CnT-PR-F-HC does not contain serum. We recommend the use of 1 % serum to deliver improved isolation efficiency and proliferation of human and animal fibroblasts in comparison with traditional 10% serum media.CnT-PR-F-HC is a highly refined medium for fibroblast culture. It uses an advanced basal medium supplemented with a range of growth factors, thereby reducing the serum requirement down to 1%.
The reduction to a 1% serum medium is achieved through the addition of a range of fully defined growth factors and co-factors to improve their binding to cell surface receptors. This combination enables more rapid expansion than traditional 10% serum formulations.
By reducing the dependence on serum, CnT-PR-F-HC is significantly more consistent than traditional high-serum formulations.
For induction of other cell behaviours such as ECM secretion, the CnT-PR-ECM medium is recommended.Thawing, seeding, and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.
The medium undergoes a range of QC tests in our lab before being released for sale.
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| Title | Year | |
|---|---|---|
| CELLnTEC Catalog | 2023 | Download |