Encouraging high secretion of extracellular matrix proteins from stromal cells in a clinically ready formulation.
| Catalog | CnT-PR-ECM-HC |
|---|---|
| Content | 500 ml, Frozen medium |
CnT-PR-ECM-HC is a fully defined medium that triggers high ECM production by fibroblasts isolated from a range of tissues. It is ideally suited for use during product development, protocol validation, and characterization of the cell-based product.
Fibroblasts growing in CnT-PR-ECM deliver 5x more collagen than proliferative cells growing in CnT-PR-F.
CnT-PR-ECM-HC is a fully defined medium. It is supplemented with a range of growth factors and vitamin C. CnT-PR-ECM-HC enables cells to produce in-vivo like quantities of ECM as a result of its altered growth factor mix (which shifts the focus away from strong proliferation), and the presence of vitamin C.When evaluating collagen secretion, it is strongly recommended to evaluate total collagen using in-well lysis of all the cells and deposited ECM (as opposed to evaluation of only the supernatant), and to normalize the results for cell number using gDNA.
A complete protocol is available for download.
The medium undergoes a range of QC tests in our lab before being released for sale. Please see the datasheet for details.
Thawing, seeding, and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.
Like to try a test sample? Click here for more information.
| Title | Year | |
|---|---|---|
| CELLnTEC Catalog | 2023 | Download |