CELLnTEC
CELLnTEC / Products / CnT-PR-ECM-HC

CnT-PR-ECM-HC

  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-Prime Fibroblast ECM Medium, Higher Certified

Encouraging high secretion of extracellular matrix proteins from stromal cells in a clinically ready formulation.

Catalog CnT-PR-ECM-HC
Content 250 ml, Frozen medium

HIGHLIGHTS:

  • Chemically defined, contains no components of animal or human origin
  • Specifically designed to encourage secretion of ECM proteins
  • Fully supplemented, one bottle ready-to-use
  • Extended QC, documentation, support, and process control for clinical researchers

Description

CnT-PR-ECM-HC is a fully defined medium that triggers high ECM production by fibroblasts isolated from a range of tissues. It is ideally suited for use during product development, protocol validation, and characterization of the cell-based product.

Back to top

Specifications

Tissue type
Fibroblasts
Species
Human, may also work for other species
Application
Differentiation
Serum
No
BPE
No
Free of Human & Animal Component
Yes
Chemically defined
Yes
Clinically upgradable
Yes
Volume
250 ml
Component(s)
Frozen Bottle & Ready-to-use
Quality level
Higher Certified

Back to top

Scientific resources

Isolation Efficiency

Fibroblasts growing in CnT-PR-ECM deliver 5x more collagen than proliferative cells growing in CnT-PR-F.

CnT-PR-ECM-HC is a fully defined medium. It is supplemented with a range of growth factors and vitamin C. CnT-PR-ECM-HC enables cells to produce in-vivo like quantities of ECM as a result of its altered growth factor mix (which shifts the focus away from strong proliferation), and the presence of vitamin C.

When evaluating collagen secretion, it is strongly recommended to evaluate total collagen using in-well lysis of all the cells and deposited ECM (as opposed to evaluation of only the supernatant), and to normalize the results for cell number using gDNA.

A complete protocol is available for download.
 

The medium undergoes a range of QC tests in our lab before being released for sale. Please see the datasheet for details.

Thawing, seeding, and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.

Like to try a test sample? Click here for more information.

Back to top

Scientific Literature

Title Year
CELLnTEC Catalog 2020 Download

Back to top