Spontaneously immortalized human bladder epithelial cells, cryo-preserved at approx. passage 25.
Catalog |
HBLAK |
Content |
Vial containing 0.5 million viable cells, frozen |
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HIGHLIGHTS:
- Extended lifespan of the cells and guarantee of at least 6 months of growth post-delivery
- Ability to complete complex processes such as 3D differentiation
- Pre-equilibrated in CELLnTEC base chemistry
- Supplied in fully-defined cryopreservation medium to maximize recovery after thawing
Description
CELLnTEC’s HBLAK long-term human bladder epithelial cells are spontaneously transformed cells that provide the convenience of long-term cell growth without senescence.
HANDLING INSTRUCTION: This material is of human origin and should therefore be handled as hazardous. Although it has been tested negative for HIV 1, Hepatitis B, and Hepatitis C, treat this material as potentially infectious and use appropriate biocontainment, protective equipment, and other precautions to prevent accidental exposure.
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Specifications
- Class
- Cell lines
- Tissue type
- Bladder
- Cell type
- Bladder Epithelium Progenitors
- Species
- Human
- Pack size
- 0.5 million viable cells (1 mL), frozen vial
- Culture Medium
- CnT-PR
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Scientific resources
HBLAK long-term human bladder epithelial cells have been isolated from normal tissue and have not been actively transformed. These cells retain the ability to differentiate and form 3D cultures.
CELLnTEC’s Long-Term cells are provided at approx. passage 25 and come with a guarantee of at least 6 months of growth post-delivery.
As with all cell cultures that deliver long-term growth, it is recommended to expand the cells after initial delivery, then freeze-down a group of stock vials. Experiments should then be conducted in the subsequent approx. 15-20 passages after thawing each of the stock vials.
Once 15-20 passages have been completed, the culture should be discarded and a new culture started from a fresh stock vial.
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Scientific Literature
Title |
Authors |
Year |
Tissue type |
A urine-dependent human urothelial organoid offers a potential alternative to rodent models of infection |
Harry Horsley, Dhanuson Dharmasena, James Malone-Lee & Jennifer L. Rohn |
2017 |
Bladder |
Effects of novel HDAC inhibitors on urothelial carcinoma cells |
Aline Kaletsch, Maria Pinkerneil, Michèle J. Hoffmann, Ananda A. Jaguva Vasudevan, Chenyin Wang, Finn K. Hansen, Constanze Wiek, Helmut Hanenberg, Christoph Gertzen, Holger Gohlke, Matthias U. Kassack, Thomas Kurz, Wolfgang A. Schulz, Günter Niegisch |
2018 |
Bladder |
Epigenetic Treatment of Urothelial Carcinoma Cells Sensitizes to Cisplatin Chemotherapy and PARP Inhibitor Treatment |
Sophia Thy, Alexandra Hommel, Sarah Meneceur, Anna L. Bartkowiak, Wolfgang A. Schulz, Günter Niegisch, Michèle J. Hoffmann |
2021 |
Bladder |
Title |
Year |
CELLnTEC Catalog |
2023 |
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