CELLnTEC

CELLnTEC’s HBLAK long-term human bladder epithelial cells are spontaneously transformed cells that provide the convenience of long-term cell growth without senescence.

These cells have been isolated from normal tissue and have not been actively transformed.

The HBLAK cells retain the ability to differentiate and form 3D cultures.

Long-Term cells are provided at approx passage 25, and come with a guarantee of at least 6 months of growth post delivery.

As with all cell cultures that deliver long-term growth, it is recommended to expand the cells after initial delivery, then freeze-down a group of stock vials. Experiments should then be conducted in the subsequent approx. 15-20 passages after thawing each of the stock vials.

Once 15-20 passages have been completed, the culture should be discarded, and a new culture started from a fresh stock vial.

Printable datasheet

Bladder Epithelium Progenitors, Long-Term

Spontaenously immortalised human bladder epithelial cells, cryo preserved at approx P25.

This material is of human origin and should therefore be handled as hazardous. Although it has been tested negative for HIV 1, Hepatitis B and Hepatitis C, treat this material as potentially infectious, and use appropriate biocontainment, protective equipment and other precautions to prevent accidental exposure.
Species
Human, single donor, bladder
Tissue type
Epithelia
Pack size
HBLAK is a vial containing > 5 x 105 cells (1 mL)
Defined
Yes
ACF
No
Culture medium
CnT-PR
Cultivation
For cultivation instructions, please see general cultivation protocol in the resources section of www.cellntec.com
Passaging
Recommended seeding density after passaging: 4 x 103 cells / cm2. For detailed passaging instructions, please see general cultivation protocol in the resources section of www.cellntec.com
Av. time to confluence
8 days after thawing, then approx 7 days subsequently (depending on temperature, seeding density and protocol)
Longevity
Guaranteed to provide at least 6 months further growth when grown in CnT-PR medium.
Storage / Shelf life
Immediately upon arrival, transfer the cryo vial to the liquid nitrogen container until ready to use. Medium storage: refer to medium label and data sheet.
Thawing
Recommended seeding density after thawing: 4,000 cells cm2. For thawing instructions, please see general cultivation protocol in the resources section of www.cellntec.com
Freezing
Recommended freezing density: 1 x 106 cells / mL. For freezing instructions, please see general cultivation protocol in the resources section of www.cellntec.com
Quality control
Tested negative for Hepatitis B, Hepatitis C and HIV-1. Free of bacteria, fungi and mycoplasma contamination.
Shipping condition
Cells are shipped on dry ice.
Comments
Bladder cells are sensitive to trypsin and trypsin like enzymes. The use of these enzymes impairs the cell attachment and cell growth, therefore CELLnTEC recommends the use of Accutase for detachement. For routine cell cultivation CELLnTEC recommends to work without antibiotics / antimycotics.
Intended use
For research use only. Not for use in therapy or diagnostics.
Last update
2019-09-20