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HBLAK

CnT Bladder Epithelium Progenitors, Adult, Long-Term

Spontaneously immortalized human bladder epithelial cells, cryo-preserved at approx. passage 25.

Catalog HBLAK
Content Vial containing 0.5 million viable cells, frozen

HIGHLIGHTS:

  • Extended lifespan of the cells and guarantee of at least 6 months of growth post-delivery
  • Ability to complete complex processes such as 3D differentiation
  • Pre-equilibrated in CELLnTEC base chemistry
  • Supplied in fully-defined cryopreservation medium to maximize recovery after thawing

Description

CELLnTEC’s HBLAK long-term human bladder epithelial cells are spontaneously transformed cells that provide the convenience of long-term cell growth without senescence. HANDLING INSTRUCTION: This material is of human origin and should therefore be handled as hazardous. Although it has been tested negative for HIV 1, Hepatitis B, and Hepatitis C, treat this material as potentially infectious and use appropriate biocontainment, protective equipment, and other precautions to prevent accidental exposure.

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Specifications

Class
Cell lines
Tissue type
Bladder
Cell type
Bladder Epithelium Progenitors
Species
Human
Pack size
0.5 million viable cells (1 mL), frozen vial
Culture Medium
CnT-PR

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Scientific resources

HBLAK long-term human bladder epithelial cells have been isolated from normal tissue and have not been actively transformed. These cells retain the ability to differentiate and form 3D cultures.

CELLnTEC’s Long-Term cells are provided at approx. passage 25 and come with a guarantee of at least 6 months of growth post-delivery.

As with all cell cultures that deliver long-term growth, it is recommended to expand the cells after initial delivery, then freeze-down a group of stock vials. Experiments should then be conducted in the subsequent approx. 15-20 passages after thawing each of the stock vials.

Once 15-20 passages have been completed, the culture should be discarded and a new culture started from a fresh stock vial.


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Scientific Literature

Title Authors Year Tissue type
A urine-dependent human urothelial organoid offers a potential alternative to rodent models of infection Harry Horsley, Dhanuson Dharmasena, James Malone-Lee & Jennifer L. Rohn 2017 Bladder
Effects of novel HDAC inhibitors on urothelial carcinoma cells Aline Kaletsch, Maria Pinkerneil, Michèle J. Hoffmann, Ananda A. Jaguva Vasudevan, Chenyin Wang, Finn K. Hansen, Constanze Wiek, Helmut Hanenberg, Christoph Gertzen, Holger Gohlke, Matthias U. Kassack, Thomas Kurz, Wolfgang A. Schulz, Günter Niegisch 2018 Bladder
Epigenetic Treatment of Urothelial Carcinoma Cells Sensitizes to Cisplatin Chemotherapy and PARP Inhibitor Treatment Sophia Thy, Alexandra Hommel, Sarah Meneceur, Anna L. Bartkowiak, Wolfgang A. Schulz, Günter Niegisch, Michèle J. Hoffmann 2021 Bladder
Title Year
CELLnTEC Catalog 2023 Download

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