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CnT-Accutase-100

  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-Accutase Cell Detachment Solution

Our recommended enzyme for cell detachment and passaging due to its gentle action and the fact that no separate reagent is required to stop the reaction (simply dilute, then centrifuge).

Catalog CnT-Accutase-100
Content 100 ml ready-to-use, Frozen

HIGHLIGHTS:

  • Ready-to-use, no separate reagent required
  • Gentle on cells
  • Effective in detaching fibroblasts, epithelial cells, and a whole range of other cell types

Description

Accutase® is a ready-to-use cell detachment solution that contains proteoloytic and collagenolytic enzymes. It is of invertebrate origin (crabs), and is free of mammalian and bacterial derived proteins. Accutase can serve as a direct replacement for trypsin.

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Specifications

Application
Isolation
Free of Human & Animal Component
No
Chemically defined
Yes
Clinically upgradable
No
Volume
100 ml
Quality level
Research Grade

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Scientific resources

Accutase® is our recommended enzyme for cell detachment and passaging due to its gentle action and the fact that no separate reagent is required to stop the reaction (simply dilute, then centrifuge).

It can be used as a direct replacement for trypsin solution, an aggressive enzyme that is demanding to use due to the very small window between effective detachment and over digestion.

Accutase is used both during isolation and also for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware. It has been proven effective in detaching: fibroblasts, epithelial cells, and a whole range of other cell types.

For recommended isolation and passaging protocols, please visit our Protocols page.

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Scientific Literature

Title Authors Year Tissue type
Engineering human cell spheroids to model embryonic tissue fusion in vitro David G. Belair, Cynthia J. Wolf, Carmen Wood, Hongzu Ren, Rachel Grindstaff, William Padgett, Adam Swank, Denise MacMillan, Anna Fisher, Witold Winnik, Barbara D. Abbott 2017 Epidermal
IRF2 is a master regulator of human keratinocyte stem cell fate Nicolas Mercado, Gabi Schutzius, Christian Kolter, David Estoppey, Sebastian Bergling, Guglielmo Roma, Caroline Gubser Keller, Florian Nigsch, Adrian Salathe, Remi Terranova, John Reece-Hoyes, John Alford, Carsten Russ, Judith Knehr, Dominic Hoepfner, Alexandra Aebi, Heinz Ruffner, Tanner C. Beck, Sajjeev Jagannathan, Calla M. Olson, Hadley E. Sheppard, Selma Z. Elsarrag, Tewis Bouwmeester, Mathias Frederiksen, Felix Lohmann, Charles Y. Lin, Susan Kirkland 2019 Epidermal

 

Title Year
CELLnTEC Catalog 2020 Download

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