- Ready-to-use, no separate reagent required
- Gentle on cells
- Effective in detaching fibroblasts, epithelial cells, and a whole range of other cell types
Our recommended enzyme for cell detachment and passaging due to its gentle action and the fact that no separate reagent is required to stop the reaction (simply dilute, then centrifuge).
|Content||100 ml ready-to-use, Frozen|
Accutase® is a ready-to-use cell detachment solution that contains proteoloytic and collagenolytic enzymes. It is of invertebrate origin (crabs), and is free of mammalian and bacterial derived proteins. Accutase can serve as a direct replacement for trypsin.
Accutase® is our recommended enzyme for cell detachment and passaging due to its gentle action and the fact that no separate reagent is required to stop the reaction (simply dilute, then centrifuge).
It can be used as a direct replacement for trypsin solution, an aggressive enzyme that is demanding to use due to the very small window between effective detachment and over digestion.
Accutase is used both during isolation and also for the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware. It has been proven effective in detaching: fibroblasts, epithelial cells, and a whole range of other cell types.
For recommended isolation and passaging protocols, please visit our Protocols page.
|Engineering human cell spheroids to model embryonic tissue fusion in vitro||David G. Belair, Cynthia J. Wolf, Carmen Wood, Hongzu Ren, Rachel Grindstaff, William Padgett, Adam Swank, Denise MacMillan, Anna Fisher, Witold Winnik, Barbara D. Abbott||2017||Epidermal|
|Human Gingival Epithelial Growth In Vitro on a Polymer‐Infiltrated Ceramic Network Restorative Material||Martin J. Smallidge, Jennifer V. Sabol, Cynthia Aita‐Holmes, Henry Chuang, Douglas P. Dickinson||2019||Oral|
|IRF2 is a master regulator of human keratinocyte stem cell fate||Nicolas Mercado, Gabi Schutzius, Christian Kolter, David Estoppey, Sebastian Bergling, Guglielmo Roma, Caroline Gubser Keller, Florian Nigsch, Adrian Salathe, Remi Terranova, John Reece-Hoyes, John Alford, Carsten Russ, Judith Knehr, Dominic Hoepfner, Alexandra Aebi, Heinz Ruffner, Tanner C. Beck, Sajjeev Jagannathan, Calla M. Olson, Hadley E. Sheppard, Selma Z. Elsarrag, Tewis Bouwmeester, Mathias Frederiksen, Felix Lohmann, Charles Y. Lin, Susan Kirkland||2019||Epidermal|