CnT-Accutase-100

Features

Accutase® is our recommended enzyme for cell detachment and passaging due to its gentle action, and the fact that no separate reagent is required to stop the reaction (simply dilute, then centrifuge).

It can be used as a direct replacement for trypsin solution, an agressive enzyme that is demanding to use due to the very small window between effective detachment and overdigestion.

Accutase is used both during isolation, and also for the routine detachment of cells from standard tissue culture plastic ware and adhesion coated plastic ware, and has been proven effective in detaching: fibroblasts, epithelial cells and a whole range of other cell types.

For recommended isolation and passaging protocols, please visit our Protocols page.

Printable datasheet

Cell Detachment Solution

Accutase® is a ready-to-use cell detachment solution, that contains proteoloytic and collagenolytic enzymes. If is of invertebrate origin (crabs), and is free of mammalian and bacterial derived proteins. Accutase can serve as a direct replacement for trypsin.

Tissue type

Enzymes

Pack size

100 mL ready-to-use Accutase solution, frozen

Defined

No

ACF

No

Storage / Shelf life

Store at -20°C. See label for expiry date. Once thawed Accutase can be stored for two months at 4°C. Accutase begins to inactivate as temperature increases.  Thaw at 4°C, and avoid repeated freeze and thaw cycles.  For passaging, pre-warm only the amount necessary to room temperature just prior to use.

Thawing

Thaw at 4°C. Do not thaw in warm water bath. Loss of enzymatic activity may occur at temperatures above 20°C.

Quality control

Tested for sterility, pH 6.8 to 7.8.

Shipping condition

CnT-Accutase-100 is shipped frozen.

Comments

Solution in DPBS without Ca²⁺ and Mg²⁺, with 0.5 mM EDTA and 3 mg / L Phenol Red

Intended use

For research use only. Not for use in therapy or diagnostics.

Activity concentration

500 to 720 U / mL / 1 x Accutase

Last update

2014-03-21