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PR2D-CC

CnT-2D Epithelial/Stromal Co-Culture Starter Kit, with Cells

Provides all the components required for establishing balanced co-culture of primary keratinocytes and fibroblasts in a fully defined environment.

Catalog PR2D-CC
Content HPEKp & HDFp cells, CnT-PR, CnT-PR-F & CnT-PR-CC media

HIGHLIGHTS:

  • Kit contains precision media specially formulated for both proliferation and balanced co-culture
  • Cost effective and convenient kit, contains all required products
  • All kit components are fully compatible, ensuring reliable cell behaviour and modelling accuracy

Description

The 2D Epithelial/Stromal Co-Culture Starter Kit (PR2D-CC) provides all the components required for establishing balanced co-culture of primary keratinocytes and fibroblasts in a fully defined environment. The kit includes primary human keratinocyte and fibroblast cells, CnT-PR and CnT-PR-F media for monolayer culture and CnT-PR-CC co-culture medium. The kit is designed to enable you to establish 2D co-culture models in your lab with ease and fidelity.

Note: The cells are of human origin and should therefore be handled as hazardous. Although it has been tested negative for HIV 1, Hepatitis B and Hepatitis C, treat this material as potentially infectious, and use appropriate biocontainment, protective equipment and other precautions to prevent accidental exposure.

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Specifications

Volume
500 ml media (2x), 250 ml media (1x), 1 ml frozen cells (2x)
Component(s)
HPEKp & HDFp cells, CnT-PR, CnT-PR-F & CnT-PR-CC media

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Scientific resources

Balance 2

Balanced co-culture of keratinocytes & fibroblasts obtained by PR2D-CC starter kit

The Keratinocyte/Fibroblast 2D Co-culture Starter Kit (PR2D-CC) enables you to establish 2D co-culture models in your lab with ease and fidelity.
 
The primary keratinocytes (HPEKp) and fibroblasts (HDFp) are initially expanded in their respective proliferation media following our recommended general protocol

 
The HPEKp and HDFp cells are further seeded at equivalent ratio into standard 2D culture with CnT-PR-CC co-culture medium, wherein they proliferate together as balanced co-culture.
 
The CnT-PR-CC is serum and BPE free, omits fibroblast inhibitory elements and thereby encourages fibroblast growth in parallel to the keratinocyte in a fully defined environment. At confluency, the 2D cultures display the characteristic small and tight cobblestone morphology of keratinocyte progenitor cell colonies, surrounded by the fibroblast cells.

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Scientific Literature

Title Year
CELLnTEC Catalog 2020 Download

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