CELLnTEC

The establishment of full thickness skin models (3D co-culture) requires primary human keratinocytes and fibroblasts.

The Full Thickness Primary Cell Value Pack (#CnT-FT-CELLS) contains both HPEK primary keratinocytes and HDF primary fibroblasts, that have been used successfully with the CnT-PR-F and CnT-PR-FTAL media to establish fully thickness skin models.

Prior to seeding for the establishment of FT models, the cells should be expanded for 1-2 passages in the following media:

HPEK keratinocytes: CnT-PR medium
HDF fibroblasts: CnT-PR-F medium

The thawing, seeding and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.

Printable datasheet

Full Thickness Primary Cell Value Pack

A value pack containing two cell vials (i) Normal human epidermal keratinocyte progenitor cells (#HPEKp), isolated from juvenile donor skin, and (ii) normal human dermal fibroblasts (#HDFp), isolated from juvenile skin.

This material is of human origin and should therefore be handled as hazardous. Although it has been tested negative for HIV 1, Hepatitis B and Hepatitis C, treat this material as potentially infectious, and use appropriate biocontainment, protective equipment and other precautions to prevent accidental exposure.
Species
Human, juvenile
Tissue type
Epithelia
Pack size
Two vials, each containing > 5 x 105 viable cells (1 mL)
Defined
Yes
ACF
No
Culture medium
HPEK: expand in CnT-PR medium. HDF: expand in CnT-PR-F medium.
Cultivation
For cultivation instructions, please see general cultivation protocol, on our resources section.
Passaging
Recommended seeding density after passaging of HPEK: 4 x 103 cells / cm2. Recommended seeding density after passaging of HDF: 1 x 103 cells / cm2. For passaging instructions, please see general cultivation protocol in the resources section of www.cellntec.com
Av. time to confluence
5 to 7 days (depending on temperature, seeding density and protocol)
Longevity
For the establishment of FT models, use within 2 passages of thawing to ensure maximum functionality.
Storage / Shelf life
Immediately upon arrival transfer the cryo vial to the liquid nitrogen container, until ready to use.
Thawing
Recommended seeding density after thawing HPEK: 4 x 103 cells / cm2. Recommended seeding density after thawing HDF: 1 x 103 cells / cm2. For thawing instructions, pleplease see general cultivation protocol in the resources section of www.cellntec.com
Freezing
Recommended freezing density: 1 x 106 cells / mL. For freezing instructions, please see general cultivation protocol in the resources section of www.cellntec.com
Quality control
Tested negative for Hepatitis B, Hepatitis C and HIV-1. Free of bacteria, fungi and mycoplasma contamination.
Shipping condition
Cells are shipped on dry ice.
Comments
For routine cell cultivation CELLnTEC recommends to work without antibiotics / antimycotics.
Intended use
For research use only. Not for use in therapy or diagnostics.
Last update
2019-07-23