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CnT-DNP-10

  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-Dispase II, Neutral Protease

An optimal emzyme for isolation of primary epithelial cells.

Catalog CnT-DNP-10
Content 5 x 100 mg (approx) powder aliquots (separate tubes)

HIGHLIGHTS:

  • Gentle but effective separation of epithelial and stromal cells, allowing populations of high purity to be seeded into the primary culture

Description

DispaseĀ® is a neutral protease that cleaves fibronectin, collagen IV, and to lesser extent collagen I, and can be used to separate epithelial tissues from underlying mesenchyme.

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Specifications

Application
Isolation
Free of Human & Animal Component
Yes
Chemically defined
No
Clinically upgradable
No
Volume
5 x 100 mg
Quality level
Research Grade

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Scientific resources

Dispase II (Neutral Protease) is an optimal enzyme for the isolation of primary epithelial cells.

It provides gentle but effective separation of epithelial and stromal cells, allowing populations of high purity to be seeded into the primary culture.

It cleaves fibronectin, collagen IV, and to lesser extent collagen I and can be used to separate epithelial tissues from underlying mesenchyme.

Our dispase product CnT-DNP-10 is provided as 5 x 100 mg (approx) powder aliquots. Powder amounts may vary from lot-to-lot, due to differing activities.

Each 100 mg aliquot is sufficient to make up 40 mL of 1x solution, which is sufficient for 2-4 isolations (depending on the quantity of tissue used). The final 1x concentration after solubilization will be 2.4 U/mL.

With 5 powder aliquots in total, CnT-DNP-10 is thus sufficient for up to 20 isolations.

For recommended isolation protocols, please see the Isolation Protocols page.

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Scientific Literature

Title Authors Year Tissue type
Elovl6 regulates mechanical damage-induced keratinocyte death and skin inflammation Yoshiyuki Nakamura, Takashi Matsuzaka, Satoko Tahara-Hanaoka, KazukoShibuya, Hitoshi Shimano, Chigusa Nakahashi-Oda, Akira Shibuya 2018 Epidermal

 

Title Year
CELLnTEC Catalog 2020 Download

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