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HPEKas

CnT-Epidermal Keratinocyte Progenitors, Adult, Single Donor

Normal human epidermal keratinocyte progenitor cells, isolated from adult donor material, cryo-preserved at early passage.

Catalog HPEKas
Content Vial containing 0.5 million viable cells, frozen

HIGHLIGHTS:

  • Extended lifespan of the cells
  • Pre-equilibrated in CELLnTEC base chemistry
  • Retention of proliferative progenitors and reduced differentiation
  • Supplied in fully-defined cryopreservation medium to maximize recovery after thawing

Description

HPEKas primary human epidermal keratinocytes (single adult donor) growing in CnT-PR medium benefit from the presence of PCT factors that aid retention of proliferative progenitors, and reduce differentiation.

HANDLING INSTRUCTION: This material is of human origin and should therefore be handled as hazardous. Although it has been tested negative for HIV 1, Hepatitis B, and Hepatitis C, treat this material as potentially infectious and use appropriate biocontainment, protective equipment, and other precautions to prevent accidental exposure.

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Specifications

Class
Primary Cells
Tissue type
Skin
Cell type
Epidermal Keratinocyte Progenitors
Donor
Adult, single donor
Species
Human
Pack size
0.5 million viable cells (1 mL), frozen vial
Culture Medium
CnT-07 / CnT-57 / CnT-PR

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Scientific resources

The culturing medium CnT-PR consists of PCT factors that aid the retention of proliferative progenitors and reduce differentiation of HPEKas cells. This extends not only the lifespan of the cells but importantly also the period during which the cells retain the ability to complete complex processes such as 3D differentiation.

The HPEK cells are provided cryopreserved in the fully defined CRYO-Defined culture medium to maximize recovery after thawing.

The cultures undergo a range of QC tests in our lab before being released for sale and are also covered by our longevity guarantee. Please see the datasheet for details.

The thawing, seeding, and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.

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Scientific Literature

Title Authors Year Tissue type
IRF2 is a master regulator of human keratinocyte stem cell fate Nicolas Mercado, Gabi Schutzius, Christian Kolter, David Estoppey, Sebastian Bergling, Guglielmo Roma, Caroline Gubser Keller, Florian Nigsch, Adrian Salathe, Remi Terranova, John Reece-Hoyes, John Alford, Carsten Russ, Judith Knehr, Dominic Hoepfner, Alexandra Aebi, Heinz Ruffner, Tanner C. Beck, Sajjeev Jagannathan, Calla M. Olson, Hadley E. Sheppard, Selma Z. Elsarrag, Tewis Bouwmeester, Mathias Frederiksen, Felix Lohmann, Charles Y. Lin, Susan Kirkland 2019 Epidermal

 
For more publications on HPEK from our literature database, see here
 
 

Title Year
CELLnTEC Catalog 2020 Download

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