3D Co-Culture: Full Thickness Skin Models
Traditional methods for preparation of full-thickness skin models depended in the use of collagen I gels for the establishment of the dermal compartment.
This significantly increases complexity, cost and variability.
Using our novel high-ECM culture media, it is now possible to establish full thickness skin models without the use of collagen gels.
The entire dermal matrix is secreted by the fibroblasts, creating a fully natural and complex mix of ECM proteins.
This approch is simpler, faster, and more reproducible. For more details, please visit the features page.
This model was recently featured at the TEDD 3R conference. Please click here to view the conference report.
Features of CnT-Prime Airlift Medium (#CnT-PR-FTAL5)
Primary keratinocytes and fibroblasts that have been tested for the establishment of FT models are also available as a separate Value Pack.
Full Thickness Primary Cell Value Pack (#CnT-FT-CELLS)
For the full written protocol describing establishing of the FT models, please visit our Protocols Page.
For more information about the reusable Spacer Plates (#CnT-SP) that increase medium volume in standard 12-well plates during air-lift culture, please click here.
Kits for Full Thickness Skin Models
Would you like the convenience of ordering a kit with everything needed to establish FT skin models in your own lab?
CELLnTEC offers two different options, one including primary keratinocytes and fibroblasts, and one without.
3D FT Starter Kit, With Primary Cells (#PRFT-CELLS-24)
3D FT Starter Kit, Without Primary Cells (#PRFT-24)