CELLnTEC / Products / CnT-57


  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-57 Epithelial Proliferation Medium, Low BPE

Efficient isolation and expansion of epithelial cells from multiple tissue types

Catalog CnT-57
Content 500 ml basal medium + supplement pack


  • Delivers enhanced performance in isolation and expansion of epithelial cells, in particular if they are difficult to grow


CnT-57 is specifically designed for isolation and expansion of epithelial cells from skin, cornea, gingiva, mammary and bladder tissue. Contains a low concentration of BPE.

Back to top


Tissue type
Epithelial cells
Human and mouse, may also work for other species
Isolation / Expansion
Free of Human & Animal Component
Chemically defined
Clinically upgradable
500 ml
Basal Media & Supplement Pack
Quality level
Research Grade

Back to top

Scientific resources

CnT-57 is a low calcium medium formulation designed for isolation and expansion of mouse and human epithelial cells. It is comprised of the CnT-07 medium supplemented with a low concentration of BPE.

It is provided as a liquid culture medium kit including both basal medium and supplements. For maximum isolation efficiency from epithelial tissues, it is recommended to supplement CnT-57 with the IsoBoost supplement (#CnT-ISO-50), which has been found to double cell yield in the first week of culture.

Primary epithelial cells growing in CnT-57 display a highly proliferative phenotype. For differentiation experiments, it is recommended to switch to the CnT-PR-D or CnT-PR-3D differentiation media.

The medium undergoes a range of QC tests in our lab before being released for sale. Please see the datasheet for details. Like to try a test sample? Click here for more information.

Thawing, seeding, and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.


Back to top

Scientific Literature

Title Authors Year Tissue type
Development of a conductive biocomposite combining graphene and amniotic membrane for replacement of the neuronal network of tissue-engineered urinary bladder J. Adamowicz, I. Pasternak, T. Kloskowski, M. Gniadek, S. V. Van Breda, M. Buhl, D. Balcerczyk, M. Gagat, D. Grzanka, W. Strupinski, M. Pokrywczynska, T. Drewa 2020 Bladder
Hypo-osmotic Stress Drives IL-33 Production in Human Keratinocytes—An Epidermal Homeostatic Response Wojciech Pietka, Denis Khnykin, Vibeke Bertelsen, Astrid Haaskjold Lossius, Tor Espen Stav-Noraas, Johanna Hol Fosse, Hilde Kanli Galtung, Guttorm Haraldsen, Olav Sundnes 2019 Epidermal
The RNA-binding protein YBX1 regulates epidermal progenitors at a posttranscriptional level Eunjeong Kwon, Kristina Todorova, Jun Wang, Rastislav Horos, Kevin K. Lee, Victor A. Neel, Gian Luca Negri, Poul H. Sorensen, Sam W. Lee, Matthias W. Hentze, Anna Mandinova 2018 Epidermal

For more publications on CnT-57 from our literature database, see here

Title Year
CELLnTEC Catalog 2020 Download

Back to top