CELLnTEC / Products / CnT-07-HC


  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-07 Epithelial Proliferation Medium, Higher Certified

Efficient isolation and expansion of epithelial cells from multiple tissue types in a clinically ready formulation.

Catalog CnT-07-HC
Content 500 ml basal medium + supplement pack


  • Market leading precision medium for isolation and expansion of epithelial cells in a clinically ready formulation
  • Chemically defined
  • Extended QC, documentation, support, and process control for clinical researchers


CnT-07-HC is a fully defined medium for mouse and human keratinocyte culture. It is ideally suited for use during product development, protocol validation, and characterization of the cell-based product.

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Tissue type
Epithelial cells
Isolation / Expansion
Free of Human & Animal Component
Chemically defined
Clinically upgradable
500 ml
Basal Medium & Supplement Pack
Quality level
Higher Certified

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Scientific resources

CnT-07- HC is a low calcium medium formulation designed for isolation and expansion of mouse and human epithelial cells. Is the original formulation from which the CnT-PR medium was developed. It is provided as a liquid culture medium kit including both basal medium and supplements.

For maximum isolation efficiency from epithelial tissues, it is recommended to supplement CnT-07-HC with the Higher Certified IsoBoost supplement (#CnT-ISO-50-HC), which has been found to double cell yield in the first week of culture.

Primary epithelial cells growing in CnT-07-HC display a highly proliferative phenotype. For differentiation experiments, it is recommended to switch to the CnT-PR-D or CnT-PR-3D differentiation media.

The medium undergoes a range of QC tests in our lab before being released for sale. Please see the datasheet for details.

Like to try a test sample? Click here for more information.

Thawing, seeding, and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.


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Scientific Literature

Title Authors Year Tissue type
Clinical Grade Human Pluripotent Stem Cell-Derived Engineered Skin Substitutes Promote Keratinocytes Wound Closure In Vitro Sophie Domingues, Annabelle Darle, Yolande Masson, Manoubia Saidani, Emilie Lagoutte, Ana Bejanariu, Julien Coutier, Raif Eren Ayata, Marielle Bouschbacher, Marc Peschanski, Gilles Lemaitre, Christine Baldeschi 2022 Epidermal
Human-Induced Pluripotent Stem Cell‒Derived Keratinocytes, a Useful Model to Identify and Explore the Pathological Phenotype of Epidermolysis Bullosa Simplex Julien Coutier, Manon Bonnette, Sabrina Martineau, Aurélie Mercadier, Sophie Domingues, Manoubia Saidani, Margot Jarrige, Hélène Polveche, Annabelle Darle, Nathalie Holic, Smail Hadj-Rabia, Christine Bodemer, Gilles Lemaitre, Cécile Martinat, Christine Baldeschi 2022 Epidermal
Title Year
CELLnTEC Catalog 2023 Download

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