CELLnTEC / Products / CnT-07-HC


  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-07 Epithelial Proliferation Medium, Higher Certified

Efficient isolation and expansion of epithelial cells from multiple tissue types in a clinically ready formulation.

Catalog CnT-07-HC
Content 500 ml basal medium + supplement pack


  • Market leading precision medium for isolation and expansion of epithelial cells in a clinically ready formulation
  • Chemically defined
  • Extended QC, documentation, support, and process control for clinical researchers


CnT-07-HC is a fully defined medium for mouse and human keratinocyte culture. It is ideally suited for use during product development, protocol validation, and characterization of the cell-based product.

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Tissue type
Epithelial cells
Human and mouse, may also work for other species
Isolation / Expansion
Free of Human & Animal Component
Chemically defined
Clinically upgradable
500 ml
Basal Medium & Supplement Pack
Quality level
Higher Certified

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Scientific resources

CnT-07- HC is a low calcium medium formulation designed for isolation and expansion of mouse and human epithelial cells. Is the original formulation from which the CnT-PR medium was developed. It is provided as a liquid culture medium kit including both basal medium and supplements.

For maximum isolation efficiency from epithelial tissues, it is recommended to supplement CnT-07-HC with the Higher Certified IsoBoost supplement (#CnT-ISO-50-HC), which has been found to double cell yield in the first week of culture.

Primary epithelial cells growing in CnT-07-HC display a highly proliferative phenotype. For differentiation experiments, it is recommended to switch to the CnT-PR-D or CnT-PR-3D differentiation media.

The medium undergoes a range of QC tests in our lab before being released for sale. Please see the datasheet for details.

Like to try a test sample? Click here for more information.

Thawing, seeding, and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.


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Scientific Literature

Title Year
CELLnTEC Catalog 2020 Download

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