CELLnTEC / Products / CnT-40-HC


  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-40 Melanocyte Proliferation Medium w/o Serum, Higher Certified

Selective isolation and rapid expansion of primary epidermal melanocytes in a clinically ready and serum-free formulation.

Catalog CnT-40-HC
Content 500 ml, Frozen medium


  • Very selective medium. Rapid growth & expansion
  • Low serum & does not contain PMA or TPA
  • Extended QC, documentation, support, and process control for clinical researchers
  • Serum-free formulation enables the end user to supplement with serum validated to deliver the desired cell behavior and known to meet the necessary regulatory requirements


CnT-40-HC is specifically designed for selective isolation and rapid expansion of primary epidermal melanocytes. It is ideally suited for use during product development, protocol validation, and characterization of the cell-based product.

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Tissue type
Isolation / Expansion
Supplementation by User
Free of Human & Animal Component
Chemically defined
Clinically upgradable
500 ml
Quality level
Higher Certified

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Scientific resources

CnT-40-HC is a serum-free formulation designed for high-efficiency isolation and expansion of primary melanocytes. The serum needs to be sourced by the user to deliver the desired cell behavior and known to meet the necessary regulatory requirements. We recommend 1 % serum supplementation.

Melanocytes growing in CnT-40-HC medium display primarily a proliferative bipolar phenotype. The cells retain melanin expression. It contains progenitor cell-targeted (PCT) factors to help retain a proliferative phenotype, plus a range of melanocyte-specific factors (including endothelin-1 and endothelin-3). It does not contain antibiotics / antimycotics, and is free of PMA and TPA.

We also offer complementary media specifically tailored for melanocyte differentiation (CnT-PR-MD) and whitening studies (CnT-PR-MH). Please see the Melanocyte Page for an overview.

The medium undergoes a range of QC tests in our lab before being released for sale. Please see the datasheet for details.

Like to try a test sample? Click here for more information.

Thawing, seeding and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.

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Scientific Literature

Title Authors Year Tissue type
Hypopigmented burn hypertrophic scar contains melanocytes that can be signaled to re-pigment by synthetic alpha-melanocyte stimulating hormone in vitro Bonnie C. Carney, Taryn E. Travis, Lauren T. Moffatt, Laura S. Johnson, Melissa M. McLawhorn, Cynthia M. Simbulan-Rosenthal, Dean S. Rosenthal, Jeffrey W. Shupp 2021 Melanocyte
Title Year
CELLnTEC Catalog 2023 Download

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