HIGHLIGHTS:
- Rapid growth & expansion of fibroblasts
- Low 1% serum formulation
- Fully supplemented, one bottle ready-to-go
- Superior performance compared to classical 10 % serum media
CnT-Prime Fibroblast Proliferation Medium
High efficiency isolation and expansion of fibroblasts, without plate coating.
| Catalog | CnT-PR-F |
|---|---|
| Content | 500 ml, Frozen medium |
Description
CnT-Prime Fibroblast is a 1% serum medium that delivers improved isolation efficiency and proliferation of human and animal fibroblasts in comparison with traditional 10% serum media.
Specifications
- Tissue type
- Fibroblasts
- Application
- Isolation / Expansion
- Serum
- Low Bovine
- BPE
- No
- Free of Human & Animal Component
- No
- Chemically defined
- No
- Clinically upgradable
- Yes
- Volume
- 500 ml
- Component(s)
- Frozen Bottle & Ready-to-use
- Quality level
- Research Grade
Scientific resources
CnT-PR-F delivers 50% more cells on day 5 post isolation compared with traditional 10% serum media.
CnT-Prime Fibroblast is a 1% serum medium that delivers improved isolation efficiency and proliferation of human and animal fibroblasts in comparison with traditional 10% serum media.CnT-Prime Fibroblast Medium is a highly refined medium for fibroblast culture. It uses an advanced basal medium supplemented with a range of growth factors, thereby reducing the serum requirement down to 1%.
The reduction to a 1% serum medium is achieved through the addition of a range of fully defined growth factors and co-factors to improve their binding to cell surface receptors. This combination enables more rapid expansion than traditional 10% serum formulations.
By reducing the dependence on serum, CnT-Prime Fibroblast is significantly more consistent than traditional high-serum formulations.
Figure: Growth rate of primary human dermal fibroblasts in the 24 days (5 passages) following isolation is 40% faster in CnT-PR-F than in the traditional CnT-05 (10% serum) medium. Cells were grown on standard tissue culture plastic, passaged with accutase, and seeded at 1’000 cells per sq. cm.
CnT-PR-F-HC is upgraded for clinical applications. Contact us at support@cellntec.com for further details and pricing.For induction of other cell behaviors such as ECM secretion, the CnT-PR-ECM medium is recommended.
The medium undergoes a range of QC tests in our lab before being released for sale. Please see the datasheet for details. It can be upgraded for clinical applications. Contact us at support@cellntec.com for further details.
Like to try a test sample? Click here for more information.
Thawing, seeding, and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.
Scientific Literature
| Title | Authors | Year | Tissue type |
|---|---|---|---|
| Bronchial thermoplasty decreases airway remodelling by blocking epithelium-derived heat shock protein-60 secretion and protein arginine methyltransferase-1 in fibroblasts | Qingzhu Sun, Lei Fang, Michael Roth, Xuemei Tang, Eleni Papakonstantinou, Weiqi Zhai, Renaud Louis, Vincent Heinen, Florence N. Schleich, Shemin Lu, Spasenjia Savic, Michael Tamm, | 2019 | Airway |
| Controlling Droplet Impact Velocity and Droplet Volume: Key Factors to Achieving High Cell Viability in Sub-Nanoliter Droplet-based Bioprinting | Wei Long Ng, Xi Huang, Viktor Shkolnikov, Guo Liang Goh, Ratima Suntornnond, Wai Yee Yeong | 2021 | Dermal |
| Introducing a new type of alternative laryngeal mucosa model | Tanja Grossmann ,Andrijana Kirsch ,Magdalena Grill,Barbara Steffan,Michael Karbiener,Luka Brcic,Barbara Darnhofer,Ruth Birner-Gruenberger,Markus Gugatschka | 2023 | Oral |
| Title | Year | |
|---|---|---|
| CELLnTEC Catalog | 2023 | Download |