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CnT-PR-F

  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-Prime Fibroblast Proliferation Medium

High efficiency isolation and expansion of fibroblasts, without plate coating.

Catalog CnT-PR-F
Content 500 ml, Frozen medium

HIGHLIGHTS:

  • Rapid growth & expansion of fibroblasts
  • Low 1% serum formulation
  • Fully supplemented, one bottle ready-to-go
  • Superior performance compared to classical 10 % serum media

Description

CnT-Prime Fibroblast is a 1% serum medium that delivers improved isolation efficiency and proliferation of human and animal fibroblasts in comparison with traditional 10% serum media.

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Specifications

Tissue type
Fibroblasts
Species
Human & Mouse
Application
Isolation / Expansion
Serum
Low Bovine
BPE
No
Free of Human & Animal Component
No
Chemically defined
No
Clinically upgradable
Yes
Volume
500 ml
Component(s)
Frozen Bottle & Ready-to-use
Quality level
Research Grade

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Scientific resources

Isolation Efficiency

CnT-PR-F delivers 50% more cells on day 5 post isolation compared with traditional 10% serum media.

CnT-Prime Fibroblast is a 1% serum medium that delivers improved isolation efficiency and proliferation of human and animal fibroblasts in comparison with traditional 10% serum media.

CnT-Prime Fibroblast Medium is a highly refined medium for fibroblast culture. It uses an advanced basal medium supplemented with a range of growth factors, thereby reducing the serum requirement down to 1%.

The reduction to a 1% serum medium is achieved through the addition of a range of fully defined growth factors and co-factors to improve their binding to cell surface receptors. This combination enables more rapid expansion than traditional 10% serum formulations.

By reducing the dependence on serum, CnT-Prime Fibroblast is significantly more consistent than traditional high-serum formulations.

ProlifRate
Figure: Growth rate of primary human dermal fibroblasts in the 24 days (5 passages) following isolation is 40% faster in CnT-PR-F than in the traditional CnT-05 (10% serum) medium. Cells were grown on standard tissue culture plastic, passaged with accutase, and seeded at 1’000 cells per sq. cm.
CnT-PR-F-HC is upgraded for clinical applications. Contact us at support@cellntec.com for further details and pricing.

For induction of other cell behaviors such as ECM secretion, the CnT-PR-ECM medium is recommended.

The medium undergoes a range of QC tests in our lab before being released for sale. Please see the datasheet for details. It can be upgraded for clinical applications. Contact us at support@cellntec.com for further details.

Like to try a test sample? Click here for more information.

Thawing, seeding, and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.

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Scientific Literature

Title Year
CELLnTEC Catalog 2020 Download

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