- Rapid growth & expansion of fibroblasts
- Low 1% serum formulation
- Fully supplemented, one bottle ready-to-go
- Superior performance compared to classical 10 % serum media
High efficiency isolation and expansion of fibroblasts, without plate coating.
|Content||500 ml, Frozen medium|
CnT-Prime Fibroblast is a 1% serum medium that delivers improved isolation efficiency and proliferation of human and animal fibroblasts in comparison with traditional 10% serum media.
CnT-Prime Fibroblast Medium is a highly refined medium for fibroblast culture. It uses an advanced basal medium supplemented with a range of growth factors, thereby reducing the serum requirement down to 1%.
The reduction to a 1% serum medium is achieved through the addition of a range of fully defined growth factors and co-factors to improve their binding to cell surface receptors. This combination enables more rapid expansion than traditional 10% serum formulations.
By reducing the dependence on serum, CnT-Prime Fibroblast is significantly more consistent than traditional high-serum formulations.
For induction of other cell behaviors such as ECM secretion, the CnT-PR-ECM medium is recommended.
The medium undergoes a range of QC tests in our lab before being released for sale. Please see the datasheet for details. It can be upgraded for clinical applications. Contact us at firstname.lastname@example.org for further details.
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Thawing, seeding, and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.
|Bronchial thermoplasty decreases airway remodelling by blocking epithelium-derived heat shock protein-60 secretion and protein arginine methyltransferase-1 in fibroblasts||Qingzhu Sun, Lei Fang, Michael Roth, Xuemei Tang, Eleni Papakonstantinou, Weiqi Zhai, Renaud Louis, Vincent Heinen, Florence N. Schleich, Shemin Lu, Spasenjia Savic, Michael Tamm,||2019||Airway|