CELLnTEC / Products / HDFs


CnT Dermal Fibroblasts, Juvenile, Single Donor

Normal human dermal fibroblasts, cryo-preserved at early passage.

Catalog HDFs
Content Vial containing 0.5 million viable cells, frozen


  • High-quality cells
  • Pre-equilibrated in CELLnTEC base chemistry
  • Ability to complete complex processes such as 3D differentiation
  • Supplied in fully-defined cryopreservation medium to maximize recovery after thawing


HDFs primary human dermal fibroblasts (single donor) deliver strong growth and extended longevity due to their juvenile origin and the strong proliferative signals provided by the CnT-PR-F medium. HANDLING INSTRUCTION: This material is of human origin and should therefore be handled as hazardous. Although it has been tested negative for HIV 1, Hepatitis B, and Hepatitis C, treat this material as potentially infectious and use appropriate biocontainment, protective equipment, and other precautions to prevent accidental exposure.

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Primary Cells
Tissue type
Cell type
Dermal Fibroblasts
Pack size
0.5 million viable cells (1 mL), frozen vial
Culture Medium

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Scientific resources

HDFs primary human dermal fibroblasts (single donor) are provided cryopreserved in the fully defined CRYO-Defined culture medium to maximize recovery after thawing.

Thawing, seeding, and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.

The cultures undergo a range of QC tests in our lab before being released for sale and are also covered by our longevity guarantee. Please see the datasheet for details.

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Scientific Literature

Title Authors Year Tissue type
A novel fully-humanised 3D skin equivalent to model early melanoma invasion Hill DS, Robinson NDP, Caley MP, Chen M, O'Toole EA, Armstrong JL, Przyborski S, Lovat PE 2015 Fibroblast
Controlling Droplet Impact Velocity and Droplet Volume: Key Factors to Achieving High Cell Viability in Sub-Nanoliter Droplet-based Bioprinting Wei Long Ng, Xi Huang, Viktor Shkolnikov, Guo Liang Goh, Ratima Suntornnond, Wai Yee Yeong 2021 Dermal
Polyvinylpyrrolidone-based bioink: influence of bioink properties on printing performance and cell proliferation during inkjet-based bioprinting Wei Long Ng, Xi Huang, Viktor Shkolnikov, Ratima Suntornnond, Wai Yee Yeong 2023 Fibroblast

For more publications on HDF from our literature database, see here

Title Year
CELLnTEC Catalog 2023 Download

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