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PR3D-K-50

CnT-3D Epithelial Starter Kit, w/o Cells

Contains the media and inserts needed to establish 3D epidermal models with improved differentiation and barrier function in your own lab.

Catalog PR3D-K-50
Content CnT-PR & CnT-PR-3D media, 48 x Cell culture inserts

HIGHLIGHTS:

  • Kit contains precision media specially formulated for both proliferation and 3D differentiation
  • Kits contains everything needed (except primary cells) to create epidermal models with high barreir functins
  • All kit components are fully compatible, ensuring reliable cell behaviour and modelling accuracy
  • Cost effective and convenient kit, contains all required products

Description

3D keratinocyte starter Kit was developed for the establishment of 3-dimensional keratinocyte cultures with improved barrier function. Kit includes CnT-Prime medium (CnT-PR) for monolayer culture, CnT-Prime 3D barrier medium (CnT-PR-3D), and 48 cell culture inserts (0.4 um pore size). Kit does not contain primary keratinocytes. Optional extra: CnT-ST-100 stain solution to evaluate confluency prior to airlift.

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Specifications

Volume
500 ml media (2x)
Component(s)
CnT-PR & CnT-PR-3D media, 48 x Cell culture inserts

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Scientific resources

Raman

Improved barrier as seen by raman spectroscopy penetration profiles.

The 3D Starter kit without primary keratinocytes (PR3D-K-50) contains the media and inserts needed to establish 3D epidermal models with improved differentiation and barrier function in your own lab.

Early passage human keratinocytes growing in CnT-PR medium are also required.

The kit contains CnT-PR proliferation medium, CnT-PR-3D barrier medium, and 48 inserts for air-lift culture. For details, please see the datasheet.

The 3D models can be routinely established using our recommended protocol.

Following this protocol, the 3D models deliver up to a 50% better barrier function than traditional media. More detailed raman spectroscopy results can be found in the following link:

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Scientific Literature

Title Year
CELLnTEC Catalog 2020 Download

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