Contains the media and inserts needed to establish 3D epidermal models with improved differentiation and barrier function in your own lab.
| Catalog | PR3D-K-50 |
|---|---|
| Content | CnT-NX-E & CnT-PR-3D media, 48 x Cell culture inserts |
3D keratinocyte starter Kit was developed for the establishment of 3-dimensional keratinocyte cultures with improved barrier function. Kit includes CnT-NX epithelial culture medium (CnT-NX-E) for monolayer culture, CnT-Prime 3D airlift medium (CnT-PR-3D), and 48 cell culture inserts (0.4 um pore size). Kit does not contain primary keratinocytes.
Improved barrier as seen by raman spectroscopy penetration profiles.
The 3D Starter kit without primary keratinocytes (PR3D-K-50) contains the media and inserts needed to establish 3D epidermal models with improved differentiation and barrier function in your own lab.
Early passage human keratinocytes growing in CnT-PR medium are also required.
The kit contains CnT-PR proliferation medium, CnT-PR-3D barrier medium, and 48 inserts for air-lift culture. For details, please see the datasheet.
The 3D models can be routinely established using our recommended protocol.
Following this protocol, the 3D models deliver up to a 50% better barrier function than traditional media. More detailed raman spectroscopy results can be found in the following link:
| Title | Year | |
|---|---|---|
| CELLnTEC Catalog | 2023 | Download |