- High-quality cells
- Pre-equilibrated in CELLnTEC base chemistry
- Extended lifespan of the cells
CELLnTEC’s long-term mouse keratinocytes are spontaneously transformed cells that provide the convenience of long-term cell growth without senescence.
|Content||Vial containing 0.5 million viable cells, frozen|
Mouse epidermal keratinocyte progenitor cells, cryo-preserved at approx passage 30. To obtain the guaranteed growth, cells are grown in CnT-PR medium.
MPEK-BL6 long-term mouse keratinocytes are isolated from normal C57BL/6 mouse skin and have not been actively transformed. These long-term keratinocytes have been widely characterized in the literature and they retain the ability to differentiate.
CELLnTEC’s Long-Term cells are provided at approx. passage 30 and come with a guarantee of at least 6 months of growth post-delivery.
As with all cell cultures that deliver long-term growth, it is recommended to expand the cells after initial delivery, then freeze-down a group of stock vials. Experiments should then be conducted in the subsequent approx. 15-20 passages after thawing each of the stock vials.
Once 15-20 passages have been completed, the culture should be discarded and a new culture started from a fresh stock vial.
|MyD88/CD40 Genetic Adjuvant Function in Cutaneous Atypical Antigen-Presenting Cells Contributes to DNA Vaccine Immunogenicity||Matthew R. Collinson-Pautz, Kevin M. Slawin, Jonathan M. Levitt, David M. Spencer||2016||Epidermal|
|PNPLA1 is a transacylase essential for the generation of the skin barrier lipid o-O-acylceramide||Ohno Y, Kamiyama N, Nakamichi S, Kihara A||2017||Epidermal|