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PR2D-KM

CnT-2D Keratinocyte/Melanocyte Co-Culture Starter Kit, with Cells

Provides all the components required for establishing balanced co-culture of primary keratinocytes and melanocytes in a fully defined environment.

Catalog PR2D-KM
Content HPEKp & HEMas cells, CnT-PR, CnT-40 & CnT-PR-KM media

HIGHLIGHTS:

  • Kit contains precision media specially formulated for both proliferation and balanced co-culture
  • Cost effective and convenient kit, contains all required products
  • All kit components are fully compatible, ensuring reliable cell behaviour and modelling accuracy

Description

The Keratinocyte/Melanocyte 2D Co-culture Starter Kit (PR2D-KM) provides all the components required for establishing balanced co-culture of primary keratinocytes and melanocytes in a fully defined environment. The kit includes primary human keratinocyte and melanocyte cells, CnT-PR and CnT-40 media for monolayer culture and CnT-PR-KM co-culture medium. The kit is designed to enable you to establish 2D co-culture models in your lab with ease and fidelity.

Note: The cells are of human origin and should therefore be handled as hazardous. Although it has been tested negative for HIV 1, Hepatitis B and Hepatitis C, treat this material as potentially infectious, and use appropriate biocontainment, protective equipment and other precautions to prevent accidental exposure.

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Specifications

Volume
500 ml media (2x), 250 ml media (1x), 1 ml frozen cells (2x)
Component(s)
HPEKp & HEMas cells, CnT-PR, CnT-40 & CnT-PR-KM media

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Scientific resources

KM_CoCulture

Balanced co-culture of keratinocytes & melanocytes obtained by PR2D-KM starter kit

The Keratinocyte/Melanocyte 2D Co-culture Starter Kit PR2D-KM enables you to establish 2D co-culture models in your lab with ease and fidelity.
 
The primary keratinocytes and melanocytes are initially expanded in their respective proliferation media following our recommended general protocol.

 
These primary cells proliferate together when seeded at an equivalent ratio into standard 2D culture with CnT-PR-KM co-culture medium.
 
The standard keratinocyte media contain ingredients that inhibit melanocyte growth or drive immediate differentiation. Consequently, the melanocytes fail to survive in 2D culture with keratinocytes. The CnT-PR-KM co-culture medium in the kit is tailored to maintain a balanced co-culture of primary human keratinocytes and melanocytes for up to a week, in a fully defined environment.

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Scientific Literature

Title Year
CELLnTEC Catalog 2020 Download

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