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KM_CoCulture

Balanced co-culture of keratinocytes & melanocytes obtained by PR2D-KM starter kit

The Keratinocyte/Melanocyte 2D Co-culture Starter Kit PR2D-KM is designed to enable you to establish 2D co-culture models in your lab with ease and fidelity.
 
The primary keratinocytes and melanocytes are initially expanded in their respective proliferation media following our recommended general protocol

 
These primary cells proliferate together when seeded at equivalent ratio into standard 2D culture with CnT-PR-KM co-culture medium.
 
The standard keratinocyte media contain ingredients that inhibit melanocyte growth, or drive immediate differentiation. Consequently the melanocytes fail to survive in 2D culture with keratinocytes.
 
The CnT-PR-KM co-culture medium in the kit is tailored to maintain a balanced co-culture of primary human keratinocytes and melanocytes for upto a week, in a fully defined environment.

Printable datasheet

Keratinocyte/Melanocyte 2D Co-culture Starter Kit

The new PR2D-KM starter kit provides all the components required for establishing balanced co-culture of primary keratinocytes and melanocytes in a fully defined environment. The kit includes primary human keratinocyte and melanocyte cells, CnT-PR and CnT-40 media for monolayer culture and CnT-PR-KM co-culture medium.

The cells are of human origin and should therefore be handled as hazardous. Although it has been tested negative for HIV 1, Hepatitis B and Hepatitis C, treat this material as potentially infectious, and use appropriate biocontainment, protective equipment and other precautions to prevent accidental exposure.
Species
HPEKp Keratinocytes: Human, pooled (3 or more donors), juvenile. HEMas Melanocytes: Human, adult, single donor.
Tissue type
Epithelia, Melanocyte
Pack size
PR2D-KM is a kit consisting of:
  • 1 x HPEKp: Human Epidermal Keratinocyte Progenitors > 5 x 105 viable cells per vial (1 mL)
  • 1 x HEMas: Human Epidermal Melanocytes> 5 x 105 viable cells per vial (1 mL)
  • 1 x CnT-PR: 500 mL CnT-Prime Epithelial Culture Medium
  • 1 x CnT-40: 500 mL CnT-Prime Melanocyte Culture Medium
  • 1 x CnT-PR-CC: 250 mL Keratinocyte/Melanocyte Co-culture Medium
  • 1 x PR2D-KM Data sheet
Media Type
2D-Coculture
Defined
Yes
ACF
Yes
Culture medium
Media included in the kit: CnT-PR Epithelial Culture Medium, CnT-40 Melanocyte Culture Medium and CnT-PR-KM Keratinocyte/Melanocyte Co-culture Medium. All media are designed for use in a 5% CO2 atmosphere. Media must be thawed just prior to use.
Cultivation
For thawing, seeding and passaging instructions please see the general cultivation protocol in the resources section of www.cellntec.com. For routine cell cultivation, CELLnTEC does not recommend the use of antibiotics / antimycotics.
Passaging
Recommended seeding density after passaging: 4 x 103 cells / cm2. For passaging instructions, please see also general cultivation protocol, on our resources section of www.cellntec.com
Av. time to confluence
5 days for monolayer culture of keratinocytes and melanocytes (depending on temperature, seeding density and protocol).
Longevity
Keratinocytes guaranteed to provide 20 population doublings when grown in CnT-PR. Melanocytes guaranteed to provide 15 population doublings when grown in CnT-40.
Storage / Shelf life
Immediately upon arrival transfer the cryo vial to the liquid nitrogen container, until ready to use. Medium storage: -20°C. Thaw overnight at 4oC prior to use, or alternatively at room temperature for approx 5 hours. Do not thaw in a hot water bath. For expiry date of frozen medium, refer to medium label. Storage of thawed medium: 4°C in the dark, use within 6 weeks of thawing. Important: The quality of cell culture media is rapidly degraded by light. Store in the dark, and minimize all light exposure during handling.
Thawing
Recommended seeding density after thawing: 4 x 103 cells / cm2. For thawing instructions, please see also general cultivation protocol, on the resources section of www.cellntec.com
Freezing
Recommended freezing density: 1 x 106 cells / mL. For freezing instructions, please see also general cultivation protocol, on the resources section of www.cellntec.com
Quality control
Primary cells tested negative for Hepatitis B, Hepatitis C and HIV-1. Media tested free of bacteria, fungi and mycoplasma contamination.
Shipping condition
All kit components are shipped together on dry ice.
Intended use
For research use only. Not for use in therapy or diagnostics.
Last update
2019-08-07