HIGHLIGHTS:
- Chemically defined, contains no components of animal or human origin
- Specifically designed for balanced co-culture of keratinocytes and melanocytes
- Fully supplemented, one bottle ready-to-use
CnT-Prime Keratinocyte/Melanocyte Co-Culture Medium
2D co-culture of keratinocytes and melanocytes
| Catalog | CnT-PR-KM |
|---|---|
| Content | 500 ml, Frozen medium |
Description
CnT-PR-KM is specifically formulated to allow a balanced co-culture of primary human keratinocytes and melanocytes in a fully defined environment.
Specifications
- Tissue type
- Melanocytes & Keratinocytes
- Application
- Co-culture
- Serum
- No
- BPE
- No
- Free of Human & Animal Component
- Yes
- Chemically defined
- Yes
- Clinically upgradable
- Yes
- Volume
- 500 ml
- Component(s)
- Frozen Bottle & Ready-to-use
- Quality level
- Research Grade
Scientific resources
Balanced co-culture of keratinocytes & melanocytes obtained by CnT-PR-KM media
Primary keratinocytes and melanocytes are initially expanded in CnT-PR and CnT-40 media respectively. Cells are then seeded at the desired ratio (e.g. 2:1 or 5:1) into standard 2D culture, from which point both cell types proliferate.The medium undergoes a range of QC tests in our lab before being released for sale. Please see the datasheet for details. It can easily be upgraded for clinical applications. Contact us at support@cellntec.com for further details and pricing.
Like to try a test sample? Click here for more information.
Thawing, seeding, and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.
Scientific Literature
| Title | Authors | Year | Tissue type |
|---|---|---|---|
| Efficient Isolation and Functional Characterization of Niche Cells from Human Corneal Limbus | Naresh Polisetti, Lyne Sharaf, Ursula Schlötzer-Schrehardt, Günther Schlunck, Thomas Reinhard | 2022 | Corneal |
| Identification, Isolation, and Characterization of Melanocyte Precursor Cells in the Human Limbal Stroma | Shen Li, Matthias Zenkel, Friedrich E. Kruse, Andreas Gießl, Ursula Schlötzer-Schrehardt | 2022 | Corneal |
| Title | Year | |
|---|---|---|
| CELLnTEC Catalog | 2023 | Download |