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CPEK

CnT-Canine Epidermal Keratinocyte Progenitors, Long-Term

Dog epidermal keratinocytes. Long-term culture, cryo-preserved at passage 30 - 40.

Catalog CPEK
Content Vial containing 0.5 million viable cells, frozen

HIGHLIGHTS:

  • High-quality cells
  • Pre-equilibrated in CELLnTEC base chemistry
  • Guarantee of at least 6 months of growth post-delivery
  • Supplied in fully-defined cryopreservation medium to maximize recovery after thawing

Description

CELLnTEC’s long-term canine epidermal keratinocytes are spontaneously transformed cells that provide the convenience of long-term cell growth without senescence.

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Specifications

Class
Cell lines
Tissue type
Skin
Cell type
Epidermal Keratinocyte Progenitors
Donor
Dog (beagle)
Species
Dog
Pack size
0.5 million viable cells (1 mL), frozen vial
Culture Medium
CnT-09

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Scientific resources

CPEK long-term canine epidermal keratinocytes are isolated from normal tissue and have not been actively transformed.

CELLnTEC’s Long-Term cells have been widely characterized, provided at approx. passage 30 – 40, and come with a guarantee of at least 6 months of growth post-delivery.

As with all cell cultures that deliver long-term growth, it is recommended to expand the cells after initial delivery, then freeze-down a group of stock vials. Experiments should then be conducted in the subsequent approx. 15-20 passages after thawing each of the stock vials.

Once 15-20 passages have been completed, the culture should be discarded and a new culture started from a fresh stock vial.

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Scientific Literature

Title Authors Year Tissue type
Augmentation of CCL17 and CCL28 gene expression by TNF-alpha, IL-1beta, or IFN-gamma in cultured canine keratinocytes Shibata S, Maeda S, Maeda S, Chimura N, Kondo N, Fukata T. 2010 Epidermal
Foxp3+ Regulatory T Cells Associated With CCL17/CCR4 Expression in Carcinomas of Dogs Shingo Maeda, Maho Nakazawa, Mona Uchida, Ryohei Yoshitake, Takayuki Nakagawa, Ryohei Nishimura, Ryo Miyamoto, Makoto Bonkobara, Tomohiro Yonezawa, Yasuyuki Momoi 2020 Epidermal

 

Title Year
CELLnTEC Catalog 2020 Download

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