CELLnTEC / Products / CPEK


CnT Canine Epidermal Keratinocyte Progenitors, Long-Term

Dog epidermal keratinocytes. Long-term culture, cryo-preserved at passage 30 - 40.

Catalog CPEK
Content Vial containing 0.5 million viable cells, frozen


  • High-quality cells
  • Pre-equilibrated in CELLnTEC base chemistry
  • Guarantee of at least 6 months of growth post-delivery
  • Supplied in fully-defined cryopreservation medium to maximize recovery after thawing


CELLnTEC’s long-term canine epidermal keratinocytes are spontaneously transformed cells that provide the convenience of long-term cell growth without senescence.

Back to top


Cell lines
Tissue type
Cell type
Epidermal Keratinocyte Progenitors
Dog (beagle)
Pack size
0.5 million viable cells (1 mL), frozen vial
Culture Medium

Back to top

Scientific resources

CPEK long-term canine epidermal keratinocytes are isolated from normal tissue and have not been actively transformed.

CELLnTEC’s Long-Term cells have been widely characterized, provided at approx. passage 30 – 40, and come with a guarantee of at least 6 months of growth post-delivery.

As with all cell cultures that deliver long-term growth, it is recommended to expand the cells after initial delivery, then freeze-down a group of stock vials. Experiments should then be conducted in the subsequent approx. 15-20 passages after thawing each of the stock vials.

Once 15-20 passages have been completed, the culture should be discarded and a new culture started from a fresh stock vial.

Back to top

Scientific Literature

Title Authors Year Tissue type
Augmentation of CCL17 and CCL28 gene expression by TNF-alpha, IL-1beta, or IFN-gamma in cultured canine keratinocytes Shibata S, Maeda S, Maeda S, Chimura N, Kondo N, Fukata T. 2010 Epidermal
Foxp3+ Regulatory T Cells Associated With CCL17/CCR4 Expression in Carcinomas of Dogs Shingo Maeda, Maho Nakazawa, Mona Uchida, Ryohei Yoshitake, Takayuki Nakagawa, Ryohei Nishimura, Ryo Miyamoto, Makoto Bonkobara, Tomohiro Yonezawa, Yasuyuki Momoi 2020 Epidermal
Polyphenols and Cannabidiol Modulate Transcriptional Regulation of Th1/Th2 Inflammatory Genes Related to Canine Atopic Dermatitis Marcella Massimini, Elena Dalle Vedove, Benedetta Bachetti, Francesco Di Pierro, Cataldo Ribecco, Claudio D'Addario, Mariangela Pucci 2021 Epidermal
Title Year
CELLnTEC Catalog 2023 Download

Back to top