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CnT-PR-A

  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-Prime Airway Epithelial Proliferation Medium

Efficient isolation and expansion of large airway epithelial cells.

Catalog CnT-PR-A
Content 500 ml, Frozen medium

HIGHLIGHTS:

  • Market leading precision medium for isolation and expansion of large airway epithelial cells
  • Chemically defined, contains no components of animal or human origin
  • Fully supplemented, one bottle ready-to-go

Description

CnT-Prime Airway is a fully defined, human and animal-component-free medium for the isolation and expansion of large airway epithelial cells.

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Specifications

Tissue type
Airway epithelial cells
Application
Isolation / Expansion
Serum
No
BPE
No
Free of Human & Animal Component
Yes
Chemically defined
Yes
Clinically upgradable
Yes
Volume
500 ml
Component(s)
Frozen Bottle & Ready-to-use
Quality level
Research Grade

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Scientific resources

CnT-PR-A is a chemically defined, low calcium (0.07 mM) medium formulation designed for optimal isolation, expansion, and longevity of primary large airway epithelial cells. It is an evolution and refinement of our previous airway medium CnT-17.

It uses an optimized basal medium with additional trace elements, protective antioxidants, and vitamins. This basal medium is then supplemented with a range of Progenitor Cell Targeted (PCT) growth factors for efficient isolation and extended longevity, and co-factors to improve growth factor binding to membrane-bound receptors.

It is completely free of animal or human-derived components. CnT-PR-A contains purified growth factors, an optimized basal medium to increase cell proliferation, and PCT factors to maximize retention of proliferative progenitor cells, increase longevity, and minimize loss through differentiation. It does not contain phenol-red, or antibiotics / antimycotics.

Large airway cells growing in CnT-PR-A retain deliver extended growth. For differentiation experiments, it is recommended to switch to the CnT-PR-AD differentiation medium.

The medium undergoes a range of QC tests in our lab before being released for sale. Please see the datasheet for details.

It can easily be upgraded for clinical applications. Contact us at support@cellntec.com for further details and pricing.

Thawing, seeding, and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.

Like to try a test sample? Click here for more information.


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Scientific Literature

Title Authors Year Tissue type
ATriple Co-CultureModeloftheHuman RespiratoryTract to Study Immune Modulatory Effects o fLiposomes and Virosomes Rebecca A. M. Blom, Silvia T. Erni, KristõÂna KrempaskaÂ, Olivier Schaerer, R. Maarten van Dijk, Mario Amacker, Christian Moser, Sean R. R. Hall, Christophe von Garnier, Fabian Blank 2016 Airway
Consecutive Hypoxia Decreases Expression of NOTCH3, HEY1, CC10, and FOXJ1 via NKX2-1 Downregulation and Intermittent Hypoxia-Reoxygenation Increases Expression of BMP4, NOTCH1, MKI67, OCT4, and MUC5AC via HIF1A Upregulation in Human Bronchial Epithelial C Yung-Yu Yang, Chao-Ju Lin, Cheng-Chin Wang, Chieh-Min Chen, Wen-Jen Kao, Yi-Hui Chen 2020 Airway
Maximizing the relevance and reproducibility of A549 cell culture using FBS-free media Aline Chary, Katherine Groff, Andreas O. Stucki, Servane Contal, Charlotte Stoffels, Sébastien Cambier, Monita Sharma, Arno C. Gutleb, Amy J. Clippinger 2022 Airway
Title Year
CELLnTEC Catalog 2023 Download

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