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CnT-ENDO-HC

  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-ENDO Endothelial Proliferation Medium w/o Serum, Higher Certified

Class-leading proliferation of primary human endothelial cells over extended periods, all in a serum-free, clinically ready formulation.

Catalog CnT-ENDO-HC
Content 500 ml, Frozen medium

HIGHLIGHTS:

  • Longevity of cells
  • Extended QC, documentation, support, and process control for clinical researchers
  • Serum-free formulation enables the end user to supplement with serum validated to deliver the desired cell behavior and known to meet the necessary regulatory requirements

Description

CnT-ENDO-HC medium is a highly refined medium for high efficiency isolation and expansion of primary human endothelial cells. The basal medium is also supplemented with a range of Progenitor Cell Targeted (PCT) growth factors to extend longevity and co-factors to improve growth factor binding to membrane-bound receptors. It does not contain phenol-red or antibiotics/antimycotics. CnT-ENDO-HC Medium is ideally suited for use during product development, protocol validation, and characterization of the cell-based product.

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Specifications

Tissue type
Endothelial cells
Species
Human
Application
Isolation / Expansion
Serum
Supplementation by User
BPE
No
Free of Human & Animal Component
Yes
Chemically defined
Yes
Clinically upgradable
Yes
Volume
500 ml
Component(s)
Frozen Bottle & Ready-to-use
Quality level
Higher Certified

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Scientific resources

CnT-ENDO_P5_P9
Figure: Primary HUVEC cells growing in CnT-ENDO medium at Passage 5 and Passage 9.

Primary human endothelial cells growing in CnT-ENDO-HC retain enhanced proliferation and extended growth. It uses an advanced basal medium supplemented with a range of growth factors and is FBS-free. We recommend use of 1 % serum for optimal performance.

Cells demonstrate the correct localization of VE-Cadherin, a critical regulator of endothelial cell behaviour.

Functionality of the HUVEC cells is also evident in the successful completion of a tube formation assay.

Figure: Primary HUVEC cells demonstrate correct juntional localization of VE-Cadherin under both static and flow (1.4 Pa) conditions. Analysis courtesy of Georgios Stefanopoulos, Laboratory of Thermodynamics in Emerging Technologies, ETH Zurich.
Figure: Tube formation (microvascular-on-a-chip) and in vitro modeling of blood clotting by primary HUVEC cells grown in CnT-ENDO medium (upper). Red: live cell staining. Green: Fluorochrom-labelled fibrinogen. Control: cells in EGM-2 medium (lower). Image courtesy of the Schroeder Group, Experimental Haemostasis, University of Bern.
Click to enlarge image. HUVEC growing in CnT-ENDO medium offer increased proliferate rates and longevity in comparison with EGM-2 medium. Image courtesy of the Schroeder Group, Experimental Haemostasis, University of Bern.
 
For an overview of the performance of HUVEC growing in CnT-ENDO medium, please download this A4 Flyer.

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Scientific Literature

Title Year
CELLnTEC Catalog 2020 Download

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