Routine 2D Culture of CELLnTEC Primary Cells
Primary cells are sensitive, and can easily perform poorly without attention to the details or thawing, seeding, passaging and freezing. We recommend the following protocol for our full range of primary cells, in conjunction with specific info in the culture medium datasheet.
General Thawing, Cultivation and Freezing Protocol
Aging of Keratinocytes with VitroAge Medium
Keratinocytes can be aged during several weeks of culture in VitroAge (CnT-AG2).
There are several important points to ensure dependable cell performance The following protoocol is recommended.