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CnT-PR-BM.1

  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-Prime Basal Medium

a basal medium used in all the CnT-Prime range of media and containing a low calcium concentration specifically chosen for optimal long-term proliferation of epithelial progenitor cells.

Catalog CnT-PR-BM.1
Content 500 ml, Frozen medium

Description

The CnT-PR-BM.1 basal medium is used in all the CnT-Prime range of media. It contains a full range of amino acids, vitamins, and minerals. It contains a low calcium concentration (0.07 mM), that is specifically chosen for optimal long-term proliferation of epithelial progenitor cells.

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Specifications

Tissue type
Epithelial cells
Application
Isolation / Expansion
Serum
No
BPE
No
Free of Human & Animal Component
Yes
Chemically defined
Yes
Clinically upgradable
Yes
Volume
500 ml
Component(s)
Frozen Bottle & Ready-to-use
Quality level
Research Grade

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Scientific resources

CnT-Prime Basal Medium is a fully defined, low calcium (0.07 mM) basal medium formulation designed for optimal isolation, growth, and longevity of primary epithelial cells. It is completely free of animal or human-derived components. CnT-Prime Basal Medium contains a full range of amino acids, minerals, vitamins and organic compounds. It does not contain growth factors, phenol-red, or antibiotics / antimycotics. Growth factors must be added to obtain cell proliferation.

CnT-Prime Basal Medium is the basal medium formulation used during preparation of the CnT-PR, CnT-PR-D, CnT-PR-3D, CnT-PR-CA, CnT-PR-A, and CnT-PR-AD media. It does not contain growth factors, and thus can be used when cells must be starved of stimulation for a defined period prior to experimentation. This medium is designed for use in a 5% CO2 atmosphere.

For routine cell cultivation, CELLnTEC does not recommend the use of antibiotics / antimycotics. During isolation, antibiotics / antimycotics are recommended up until the end of P1.

 


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Scientific Literature

Title Authors Year Tissue type
A Three-Dimensional Organoid Culture Model to Assess the Influence of Chemicals on Morphogenetic Fusion A Three-Dimensional Organoid Culture Model to Assess the Influence of Chemicals on Morphogenetic Fusion 2018 Epidermal
miR-24 controls the regenerative competence of hair follicle progenitors by targeting Plk3 Fengzhen Liu, Xia Zhang, You Peng, Liping Zhang, Yao Yu, Peng Hua, Peiying Zhu, Xinyu Yan, Yin Li, Liang Zhang 2021 Epidermal
Tumor Architecture and Notch Signaling Modulate Drug Response in Basal Cell Carcinoma Markus Eberl, Doris Mangelberger, Jacob B. Swanson, Monique E. Verhaegen, Paul W. Harms, Marcus L. Frohm, Andrzej A. Dlugosz, Sunny Y. Wong 2018 Epidermal
Title Year
CELLnTEC Catalog 2023 Download

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