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CnT-PR-BM.1

  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-Prime Basal Medium

a basal medium used in all the CnT-Prime range of media and containing a low calcium concentration specifically chosen for optimal long-term proliferation of epithelial progenitor cells.

Catalog CnT-PR-BM.1
Content 500 ml, Frozen medium

Description

The CnT-PR-BM.1 basal medium is used in all the CnT-Prime range of media. It contains a full range of amino acids, vitamins, and minerals. It contains a low calcium concentration (0.07 mM), that is specifically chosen for optimal long-term proliferation of epithelial progenitor cells.

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Specifications

Tissue type
Epithelial cells
Species
Human, may also work for other species
Application
Isolation / Expansion
Serum
No
BPE
No
Free of Human & Animal Component
Yes
Chemically defined
Yes
Clinically upgradable
Yes
Volume
500 ml
Component(s)
Frozen Bottle & Ready-to-use
Quality level
Research Grade

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Scientific resources

CnT-Prime Basal Medium is a fully defined, low calcium (0.07 mM) basal medium formulation designed for optimal isolation, growth, and longevity of primary epithelial cells. It is completely free of animal or human-derived components. CnT-Prime Basal Medium contains a full range of amino acids, minerals, vitamins and organic compounds. It does not contain growth factors, phenol-red, or antibiotics / antimycotics. Growth factors must be added to obtain cell proliferation.

CnT-Prime Basal Medium is the basal medium formulation used during preparation of the CnT-PR, CnT-PR-D, CnT-PR-3D, CnT-PR-CA, CnT-PR-A, and CnT-PR-AD media. It does not contain growth factors, and thus can be used when cells must be starved of stimulation for a defined period prior to experimentation. This medium is designed for use in a 5% CO2 atmosphere.

For routine cell cultivation, CELLnTEC does not recommend the use of antibiotics / antimycotics. During isolation, antibiotics / antimycotics are recommended up until the end of P1.

 

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Scientific Literature

Title Authors Year Tissue type
A Three-Dimensional Organoid Culture Model to Assess the Influence of Chemicals on Morphogenetic Fusion A Three-Dimensional Organoid Culture Model to Assess the Influence of Chemicals on Morphogenetic Fusion 2018 Epidermal
Title Year
CELLnTEC Catalog 2020 Download

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