PROLIFERATION MEDIA

CnT-NX-E is the next generation successor to CnT-07 and CnT-PR, CELLnTEC’s market leading precision media for isolation and expansion of epithelial cells from skin, cornea, gingiva, mammary, and bladder tissue. This is achieved with a combination of Progenitor Cell Targeted (PCT) factors to improve retention of cells in an undifferentiated phenotype, and co-factors to improve growth factor binding and proliferation.

Double your isolation efficiency from every valuable biopsy! Try the CnT-ISO-50 supplement.

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CnT-NX-E

Primary human adult keratinocytes cultured at passage 4 in the new CnT-NX-E

CnT-NX-E Next Generation Epithelial Proliferation Medium

  • Next generation precision medium for isolation and expansion of epithelial cells
  • Supports primary cells, iPSC derived cells, and cell lines

    • Chemically defined, contains no components of animal or human origin

  • Fully supplemented, one bottle ready-to-go
  • Excellent inter-experimental and lot-to-lot consistency
  • Can be made available in a clinically ready Higher Certified version

Features of Next Generation CnT-NX-E

 
 
 
You can checkout our legacy products listed below:
CnT-PR (Prime Epithelial Proliferation Medium)
CnT-07 (Epithelial Proliferation Medium)
CnT-57 (Epithelial Proliferation Medium, Low BPE)

HPEK_2D Differentiation

Upregulation of differentiation markers during 8-days following confluence, switch to CnT-PR-D, and addition of calcium. Results courtesy Prof. My Mahoney, Dept. Dermatology, Jefferson University.

Differentiation Media

The keratinocyte differentiation process is a complex and highly-orchestrated series of events.

It is significantly affected by the cell’s environment.

Proliferation media contain a wide range of factors that are directly inhibitory to cell differentiation.

As a result, these media actively retard the differentiation process.

The new CnT-Prime 2D Differentiation medium is specifically designed to encourage complete differentiation of epidermal keratinocytes.

Following calcium switch at confluency, these cells increase expression of a wide range of differentiation markers over a period of one week (see example to the right).

Features of CnT-Prime 2D Diff. (#CnT-PR-D)

 

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For recommended differentiation protocols, please visit the Resources for more information.