EPIDERMAL 2D PROLIFERATION MEDIA

 
CELLnTEC’s finely tuned media for the isolation and culture of primary epithelial cells.
 

CnT-NX-E is the next generation successor to CnT-07 and CnT-PR, precision media for isolation and expansion of epithelial cells from skin, cornea, gingiva, mammary, and bladder tissue. This is achieved with a combination of Progenitor Cell Targeted (PCT) factors to improve retention of cells in an undifferentiated phenotype, and co-factors to improve growth factor binding and proliferation.
CnT-NX-EX is a next generation cell culture medium designed for the efficient isolation and prolonged expansion of primary epithelial cells derived from various tissue types.

Double your isolation efficiency from every valuable biopsy! Try the CnT-ISO-50 supplement.

Like to try a test sample? Click here for more information.

CnT-NX-E

Primary human adult keratinocytes cultured at passage 4 in the new CnT-NX-E

CnT-NX-E Next Generation Epithelial Proliferation Medium

    • Next generation precision medium for isolation and expansion of epithelial cells
    • Supports primary cells, iPSC derived cells, and cell lines
    • Chemically defined, contains no components of animal or human origin
    • Fully supplemented, one bottle ready-to-go
    • Excellent inter-experimental and lot-to-lot consistency

 
Learn more about highlights and features of our next-generation media:

Features of Rapid Expansion Medium: CnT-NX-E

Features of Prolonged Expansion Medium: CnT-NX-EX

 
You can checkout our legacy products listed below:
CnT-PR (Prime Epithelial Proliferation Medium)
CnT-07 (Epithelial Proliferation Medium)
CnT-57 (Epithelial Proliferation Medium, Low BPE)
 
 

PRODUCT SELECTION TABLE

Higher Certified (HC) version

Our isolation and expansion media can be made available in a clinically ready Higher Certified version. We offer of the following media: CnT-NX-E-HC, CnT-PR-HC and CnT-07-HC.

HPEK_2D Differentiation

Upregulation of differentiation markers during 8-days following confluence, switch to CnT-PR-D, and addition of calcium. Results courtesy Prof. My Mahoney, Dept. Dermatology, Jefferson University.

EPIDERMAL 2D Differentiation Media

The keratinocyte differentiation process is a complex and highly-orchestrated series of events.

It is significantly affected by the cell’s environment.

Proliferation media contain a wide range of factors that are directly inhibitory to cell differentiation.

As a result, these media actively retard the differentiation process.

The new CnT-Prime 2D Differentiation medium is specifically designed to encourage complete differentiation of epidermal keratinocytes.

Following calcium switch at confluency, these cells increase expression of a wide range of differentiation markers over a period of one week (see example to the right).

Features of CnT-Prime 2D Diff. (#CnT-PR-D)

 

Like to try a test sample? Click here for more information.

For recommended differentiation protocols, please visit the Resources for more information.