HPEK_d6

Primary human keratinocytes growing in CnT-PR medium. Day 5 after seeding.

Proliferation Media

CnT-Prime medium is carefully formulated to deliver excellent keratinocyte growth and longevity in a fully defined, animal component free environment.

This is achieved with a combination of Progenitor Cell Targeted (PCT) factors to improve retention of cells in an undifferentiated phenotype, and co-factors to improve growth factor binding and proliferation.

Features of CnT-Prime (#CnT-PR)

Double your isolation efficiency from every valuable biopsy! Try the CnT-ISO-50 supplement.

Please see Product Table (below) for associated products such as antibiotics, enzymes, biopsy media.

Like to try a test sample? Click here for more information.

Original Media such as CnT-07, CnT-57 and CnT-09 remain available.

HPEK_2D Differentiation

Upregulation of differentiation markers during 8-days following confluence, switch to CnT-PR-D, and addition of calcium. Results courtesy Prof. My Mahoney, Dept. Dermatology, Jefferson University.

Differentiation Media

The keratinocyte differentiation process is a complex and highly-orchestrated series of events.

It is significantly affected by the cell’s environment.

Proliferation media contain a wide range of factors that are directly inhibitory to cell differentiation.

As a result, these media actively retard the differentiation process.

The new CnT-Prime 2D Differentiation medium is specifically designed to encourage complete differentiation of epidermal keratinocytes.

Following calcium switch at confluency, these cells increase expression of a wide range of differentiation markers over a period of one week (see example to the right).

Features of CnT-Prime 2D Diff. (#CnT-PR-D)

Like to try a test sample? Click here for more information.

For recommended differentiation protocols, please visit the Resources for more information.

Products table

CAT# Description Species Defined ACF Media type
CnT-PR CnT-Prime, Epithelial Culture Medium Human Yes Yes 2D-Prolif
CnT-PR-D CnT-Prime 2D Diff, Epithelial Culture Medium Human Yes Yes 2D-Diff
CnT-PR-H CnT-Prime Keratinocyte Homeostasis Medium Human Yes No 2D-Diff
CnT-PR-BM.1 CnT-Prime Basal Medium Human Yes Yes 2D-Prolif
CnT-07 Epidermal Keratinocyte Medium Human and Mouse Yes No 2D-Prolif
CnT-57 Epidermal Keratinocyte Medium Human and Mouse No No 2D-Prolif
CnT-09 Epidermal Keratinocyte Medium Dog No No 2D-Prolif
CnT-ISO-50 CnT-IsoBoost Supplement Yes Yes
CnT-ABM10 Pen / Strep / Amphotericin B Solution, Single Aliquots Yes Yes
CnT-GAB10 Gentamycin / Amphotericin B Solution, Single Aliquots Yes Yes
CnT-DNP-10 Dispase II, Neutral Protease, 5 x 0.1 gram No No
CnT-Accutase-100 Cell Detachment Solution No No