Differentiation and stratification of epithelial cells in air-lift culture places divergent demands on the culture medium
The medium must send proliferative signals to retain the basal layer, but must also permit cells to fully differentiate and form an the specialized cells of the upper layers.
The Prime Airway Differentiation medium is specifically designed to deliver this delicate balance. It is able to retain the basal layer, but in parallel allows more complete differentiation.
For complete differentiation, the starting cells are particularly important. For more details abou the protocol, please visit our Protocols Page.