COVID-19 & RESPIRATORY DISEASES RESEARCH TOOLS

 

 
 
 
Balance 2
 

Novel Coronavirus (CDC Illustration #23312)

AIRWAY EPITHELIAL CELL CULTURE

 

Infectious diseases rank among the top respiratory system illnesses. Influenza and novel coronavirus-associated pandemics including SARS (2003), MERS (2015), and COVID-19 (2019) have drawn attention to viral infections. Since the identification of SARS-CoV-2 as the causative agent of COVID-19, a worldwide endeavour to study and combat this new health threat is under way.

In vitro models using primary cultures of airway epithelial cells have become pivotal tools for research of the pathogenesis of respiratory viruses. Air-liquid-interface (ALI) models epitomize in vivo phenotypes of polarization, beating cilia, barrier, and virus-host cell interactions. 3D ALI models serve as pragmatic instruments for vaccine development and identification of antiviral drug candidates.

CELLnTEC OFFERS SPECIALTY MEDIA DESIGNED FOR LARGE AIRWAY EPITHELIAL CELLS.

CELLnTEC’s market leading precision media have been widely implemented in the development of airway models for studying respiratory viruses like:

  • Rhinovirus
  • Influenza virus
  • Infectious bronchitis virus
  • Respiratory syncytial virus
LargeAirway_p2

Primary human large airway epithelial
cells proliferation in CnT-PR-A

Order Now

AIRWAY PROLIFERATION MEDIA

Isolation and expansion of large airway epithelial cells:

 

CnT-PRIME AIRWAY EPITHELIAL CULTURE MEDIUM (#CnT-PR-A)

  • Chemically defined, contains no components of animal or human origin
  • Fully supplemented, one bottle ready-to-go
  • Evolved successor medium to CnT-17

 

AIRWAY EPITHELIUM MEDIUM (#CnT-17)

  • Chemically defined
  • Liquid culture medium kit including both basal medium and supplements

 

Both, CnT-PR-A and CnT-17 offer:

  • Excellent growth and longevity of large airway epithelial cells
  • PCT factors enhance retention of cells in an undifferentiated phenotype
  • Co-factors improve growth factor binding and proliferation
Airway3D_Day20

Human large airway cells differentiation
at the air-liquid interface using CnT-PR-AD

Order Now

AIRWAY DIFFERENTIATION MEDIUM

Differentiation of large airway epithelial cells. 3D/Barrier/ALI & 2D differentiation:

 

CnT-PRIME AIRWAY DIFFERENTIATION MEDIUM (#CnT-PR-AD)

  • Chemically defined, contains no components of animal or human origin
  • Delivers differentiation and stratification of large airway epithelial cells
  • Applicable for 2D differentiation or 3D differentiation (Barrier model/ALI culture)
  • Fully supplemented, one bottle ready-to-go

PROTOCOLS:

Use our recommended protocol and streamlined workflow for large airway epithelial cells

2D Differentiation 3D Differentiation

 
 

“We have tested several commercially available media for selection and growth of human airway epithelial cells and in our hands the CnT-Prime Airway medium is the best in class”

– [Dr Sean Hall, Department of Clinical Research, University Hospital Bern]


 
 

 
 
 
 
Balance 2

The spherical coronavirus particles seen in COVID-19 patient isolate (CDC PHIL ID #23354)

ENDOTHELIAL CELL CULTURE

 

SARS-CoV-2 infects the host using the angiotensin-converting enzyme 2 (ACE2) receptor expressed in several organs and on endothelial cells that traverse their vascular beds. Endothelial injury has been observed in COVID-19 patients occuring either through direct viral infection of the endothelium or an immune-mediated response.

Clinical studies suggest that anti-inflammatory drugs stabilize the endothelium and improve endothelial function in COVID-19 patients. ACE2 inhibitors are being developed as a strategy for vulnerable patients with pre-existing endothelial dysfunction. A better mechanistic understanding of the endothelial cells is of utmost importance for future therapeutic approaches.

CNT-ENDO, CELLnTEC’s SPECIALTY MEDIUM DESIGNED FOR ENDOTHELIAL CELLS.

CELLnTEC’s class-leading medium retains longevity and high functionality of endothelial cells, measured by both marker expression, and tube formation in in vitro model of blood clotting.

CnT-ENDO_P5

HUVEC endothelial cells growing
in CnT-ENDO medium (P5)

Order Now

ENDOTHELIAL PROLIFERATION MEDIUM

Isolation and expansion of primary human endothelial cells over extended periods:

 

CnT-Endothelium MEDIUM (#CnT-ENDO)

  • FBS-free, contains no bovine-derived products
  • Fully supplemented, one bottle ready-to-go
  • Rapid expansion by retaining cells in an undifferentiated phenotype
  • Co-factors improve growth factor binding and proliferation
  • Consistent proliferation over extended passages without any coating

 

SCIENTIFIC LITERATURE

 

CELLnTEC’s AIRWAY MEDIA DEPLOYED FOR 2D/3D CULTURING OF AIRWAY CELLS FROM DIFFERENT SPECIES FOR RESPIRATORY VIRAL RESEARCH:

 

Airway Cell Culture System Respiratory Virus Article Link
Primary human airway epithelial cells SARS-CoV-2 virus Ding et al. 2022
3D ALI models of human upper and lower airway Pandemic influenza A, H1N1pdm Xia et al. 2020
Primary human bronchial epithelial cells Human rhinovirus Roth et al. 2017
Triple co-culture of human respiratory tract Influenza virosomes or liposomes Blom et al. 2016
3D airway culture from Ferret trachea Human Influenza A or B viruses Elderfield et al. 2015
3D ALI culture of human nasal epithelial cells Murine-adapted influenza virus Kumar et al. 2011
Avian tracheal epithelial cell culture system Infectious bronchitis virus Shen et al. 2010
Monolayers of murine primary airway epithelial cells Human rhinovirus Brockman-Schneider et al. 2008

 

Xia et al. 2020 used CnT-PR-AD and demonstrated that 3D ALI cultures of the human lower airway track epithelial cells expressed a higher level of viral receptor ACE2 mRNA as compared to the upper airway cells, suggesting that these 3D models are well-suited for studying SARS-CoV-2 coronavirus.
 
Bayyoud et al. 2022 used CnT-PR for isolation and culture of human corneal epithelial cells from deceased COVID-19 donors. This study concluded that cornea and limbal epithelial cells are refractory to productive SARS-CoV-2 infection and virus is not transmit from an infected corneal transplant donor to a recipient in corneal organ cultures.
 
 

3D ORGANOIDS DEVELOPED WITH CELLnTEC’s AIRWAY MEDIA FOR EVALUATING THE POTENTIAL OF CELL THERAPY TO TREAT CHRONIC RESPIRATORY DISEASES SUCH AS ASTHMA, COPD, PULMONARY AND CYSTIC FIBROSIS:

 

3D Organotypic Model Article Link
HGEPp culture and Human periodontal tissue 3D equivalents Gao et al. 2023
3D cultured human lung epithelial cells differentiated to form bronchiole-like and alveolus-like organoids Tanaka et al. 2018
3D ALI murine lung scaffolds to induce embryonic stem cells to differentiate into various types of lung cells Kawai et al. 2018

 

Balance 2

SUPPORT

 

COVID-19 RESPONSE:

CELLnTEC operates on a regular scale, amidst the COVID-19 situation

TECHNICAL SUPPORT:

Contact scientists in our R&D team for support in accelerating scientific discovery

AIRWAY MEDIA PROMOTION:

Benefit from the special discount on CnT-PR-A and CnT-PR-AD, until January 2021

Products table

CAT# Description Species Defined ACF Media type
CnT-17 CnT-17 Airway Epithelial Proliferation Medium Yes No Airway epithelial cells
CnT-PR-A CnT-Prime Airway Epithelial Proliferation Medium Yes Yes Airway epithelial cells
CnT-PR-AD CnT-Prime Airway Epithelial 2D/3D Differentiation Medium Yes Yes Airway epithelial cells
CnT-ENDO CnT-ENDO Endothelial Proliferation Medium No No Endothelial cells
CnT-ENDO-HC CnT-ENDO Endothelial Proliferation Medium w/o Serum, Higher Certified Yes Yes Endothelial cells
CnT-ABM-10 CnT Pen-Strep / Amphotericin B Solution, 250 x, Ready-to-Use Single Aliquots Yes Yes
CnT-GAB-10 CnT Gentamycin / Amphotericin B Solution, 500 x, Ready-to-Use Single Aliquots Yes Yes
CnT-DNP-10 CnT Dispase II, Neutral Protease No Yes