COVID-19 & RESPIRATORY DISEASES RESEARCH TOOLS

 

 
 
 
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Novel Coronavirus (CDC Illustration #23312)

AIRWAY EPITHELIAL CELL CULTURE

 

Infectious diseases rank among the top respiratory system illnesses. Influenza and novel coronavirus-associated pandemics including SARS (2003), MERS (2015), and COVID-19 (2019) have drawn attention to viral infections. Since the identification of SARS-CoV-2 as the causative agent of COVID-19, a worldwide endeavour to study and combat this new health threat is under way.

In vitro models using primary cultures of airway epithelial cells have become pivotal tools for research of the pathogenesis of respiratory viruses. Air-liquid-interface (ALI) models epitomize in vivo phenotypes of polarization, beating cilia, barrier, and virus-host cell interactions. 3D ALI models serve as pragmatic instruments for vaccine development and identification of antiviral drug candidates.

CELLnTEC OFFERS SPECIALTY MEDIA DESIGNED FOR LARGE AIRWAY EPITHELIAL CELLS.

CELLnTEC’s market leading precision media have been widely implemented in the development of airway models for studying respiratory viruses like:

  • Rhinovirus
  • Influenza virus
  • Infectious bronchitis virus
  • Respiratory syncytial virus
LargeAirway_p2

Primary human large airway epithelial
cells proliferation in CnT-PR-A

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AIRWAY PROLIFERATION MEDIA

Isolation and expansion of large airway epithelial cells:

  • Chemically defined, contains no components of animal or human origin
  • Fully supplemented, one bottle ready-to-go
  • Evolved successor medium to CnT-17

Isolation and expansion of large airway epithelial cells:

  • Chemically defined
  • Liquid culture medium kit including both basal medium and supplements

 

Both, CnT-PR-A and CnT-17 offer:

  • Excellent growth and longevity of large airway epithelial cells
  • PCT factors enhance retention of cells in an undifferentiated phenotype
  • Co-factors improve growth factor binding and proliferation
Airway3D_Day20

Human large airway cells differentiation
at the air-liquid interface using CnT-PR-AD

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AIRWAY DIFFERENTIATION MEDIUM

Differentiation of large airway epithelial cells. 3D/Barrier/ALI & 2D differentiation:

  • Chemically defined, contains no components of animal or human origin
  • Delivers differentiation and stratification of large airway epithelial cells
  • Applicable for 2D differentiation or 3D differentiation (Barrier model/ALI culture)
  • Fully supplemented, one bottle ready-to-go

PROTOCOLS:

Use our recommended protocol and streamlined workflow for large airway epithelial cells

 
 

“We have tested several commercially available media for selection and growth of human airway epithelial cells and in our hands the CnT-Prime Airway medium is the best in class”

– [Dr Sean Hall, Department of Clinical Research, University Hospital Bern]


 
 

 
 
 
 
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The spherical coronavirus particles seen in COVID-19 patient isolate (CDC PHIL ID #23354)

ENDOTHELIAL CELL CULTURE

 

SARS-CoV-2 infects the host using the angiotensin-converting enzyme 2 (ACE2) receptor expressed in several organs and on endothelial cells that traverse their vascular beds. Endothelial injury has been observed in COVID-19 patients occuring either through direct viral infection of the endothelium or an immune-mediated response.

Clinical studies suggest that anti-inflammatory drugs stabilize the endothelium and improve endothelial function in COVID-19 patients. ACE2 inhibitors are being developed as a strategy for vulnerable patients with pre-existing endothelial dysfunction. A better mechanistic understanding of the endothelial cells is of utmost importance for future therapeutic approaches.

CNT-ENDO, CELLnTEC’s SPECIALTY MEDIUM DESIGNED FOR ENDOTHELIAL CELLS.

CELLnTEC’s class-leading medium retains longevity and high functionality of endothelial cells, measured by both marker expression, and tube formation in in vitro model of blood clotting.

CnT-ENDO_P5

HUVEC endothelial cells growing
in CnT-ENDO medium (P5)

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ENDOTHELIAL PROLIFERATION MEDIUM

Isolation and expansion of primary human endothelial cells over extended periods:

  • FBS-free, contains no bovine-derived products
  • Fully supplemented, one bottle ready-to-go
  • Rapid expansion by retaining cells in an undifferentiated phenotype
  • Co-factors improve growth factor binding and proliferation
  • Consistent proliferation over extended passages without any coating

SCIENTIFIC LITERATURE

 
 

CELLnTEC’s AIRWAY MEDIA DEPLOYED FOR 2D/3D CULTURING OF AIRWAY CELLS FROM DIFFERENT SPECIES FOR RESPIRATORY VIRAL RESEARCH:

 

Airway Cell Culture System Respiratory Virus Article Link
Primary human bronchial epithelial cells Human rhinovirus Roth et al. 2017
Triple co-culture of human respiratory tract Influenza virosomes or liposomes Blom et al. 2016
3D airway culture from Ferret trachea Human Influenza A or B viruses Elderfield et al. 2015
3D ALI culture of human nasal epithelial cells Murine-adapted influenza virus Kumar et al. 2011
Avian tracheal epithelial cell culture system Infectious bronchitis virus Shen et al. 2010
Monolayers of murine primary airway epithelial cells Human rhinovirus Brockman-Schneider et al. 2008

3D ORGANOIDS CREATED WITH CELLnTEC’s AIRWAY MEDIA FOR EVALUATING THE POTENTIAL OF CELL THERAPY FOR TREATING CHRONIC RESPIRATORY DISEASES SUCH AS ASTHMA, COPD, PULMONARY AND CYSTIC FIBROSIS:

 

3D Organotypic Model Article Link
3D cultured human lung epithelial cells differentiated to form bronchiole-like and alveolus-like organoids Tanaka et al. 2018
3D ALI murine lung scaffolds to induce embryonic stem cells to differentiate into various types of lung cells Kawai et al. 2018

 

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SUPPORT

 

COVID-19 RESPONSE:

CELLnTEC operates on a regular scale, amidst the COVID-19 situation

TECHNICAL SUPPORT:

Contact scientists in our R&D team for support in accelerating scientific discovery

AIRWAY MEDIA PROMOTION:

Benefit from the special discount on CnT-PR-A and CnT-PR-AD, until our stocks last

Products table

CAT# Description Species Defined ACF Media type
CnT-PR-A CnT-Prime Airway, Epithelial Culture Medium Human Yes Yes 2D-Prolif
CnT-17 Airway Epithelium Medium Human and Mouse Yes No 2D-Prolif
CnT-PR-AD CnT-Prime Airway Diff, Epithelial Culture Medium Human Yes Yes 3D-Diff
CnT-ENDO CnT-Endothelium Medium Human No No 2D-Prolif
CnT-ABM10 Pen / Strep / Amphotericin B Solution, Single Aliquots Yes Yes
CnT-DNP-10 Dispase II, Neutral Protease, 5 x 0.1 gram No No
CnT-GAB10 Gentamycin / Amphotericin B Solution, Single Aliquots Yes Yes