A microfluidic platform integrating functional vascularized organoids-on-chip
Authors
Clément Quintard, Gustav Jonsson, Camille Laporte, Caroline Bissardon, Amandine Pitaval, Nicolas Werschler, Alexandra Leopoldi, Astrid Hagelkrüys, Pierre Blandin, Jean-Luc Achard, Fabrice Navarro,Yves Fouillet, Josef M. Penninger, Xavier Gidrol
Institution
Univ.Grenoble Alpes
Country
France
Year
2024
Journal
Nature Communications
Abstract
The development of vascular networks in microfluidic chips is crucial for the
long-term culture of three-dimensional cell aggregates such as spheroids,
organoids, tumoroids, or tissue explants. Despite rapid advancement in
microvascular network systems and organoid technologies, vascularizing
organoids-on-chips remains a challenge in tissue engineering. Most existing
microfluidic devices poorly reflect the complexity of in vivo flows and require
complex technical set-ups. Considering these constraints, we develop a platform
to establish and monitor the formation of endothelial networks around
mesenchymal and pancreatic islet spheroids, as well as blood vessel organoids
generated frompluripotent stem cells, cultured for up to 30 days on-chip.We
show that these networks establish functional connections with the
endothelium-rich spheroids and vascular organoids, as they successfully provide
intravascular perfusion to these structures.We find that organoid growth,
maturation, and function are enhanced when cultured on-chip using our vascularization
method. This microphysiological system represents a viable
organ-on-chip model to vascularize diverse biological 3D tissues and sets the
stage to establish organoid perfusions using advanced microfluidics.
Product use
Mesenchymal spheroids generation with endothelial cells