CELLnTEC

Features

HGEPs primary human gingival epithelial cells (single donor) benefit from the presence of PCT factors in CELLnTEC media that aid retention of proliferative progenitors, and reduce differentiation.

The HGEP cells are provided cryopreserved in the fully defind CRYO-Defined culture medium, to maximise recovery after thawing.

The cultures undergo a range of QC tests in our lab before being released for sale, and are also covered by our longevity guarantee. Please see the datasheet for details.

CnT-57 is the recommended medium for HGEP cells for best growth and longevity. This low-BPE medium improves attachment and proliferation on standard cell culture plastic for most cell lots.

The thawing, seeding and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.

Please visit our publications database and search by tissue type to obtain a range of articles that have used CELLnTEC primary cells and media in this specific field.

Printable datasheet

Gingival Epithelium Progenitors, Single Donor

Normal human gingival progenitor cells, cryo preserved at P2.

This material is of human origin and should therefore be handled as hazardous. Although it has been tested negative for HIV 1, Hepatitis B and Hepatitis C, treat this material as potentially infectious, and use appropriate biocontainment, protective equipment and other precautions to prevent accidental exposure.
Species
Human, single donor, adult gingiva
Tissue type
Epithelia
Pack size
Vial containing > 5 x 105 viable cells (1 mL)
Defined
Yes
ACF
No
Culture medium
CnT-57 is recommended for best growth rate and longevity. CnT-PR possible for some lots, please enquire for details.
Cultivation
For cultivation instructions, please see general cultivation protocol in the resources section of www.cellntec.com
Passaging
Recommended seeding density after passaging: 4 x 103 cells / cm2 . For passaging instructions, please see general cultivation protocol in the resources section of www.cellntec.com
Av. time to confluence
5 to 7 days (depending on temperature, seeding density and protocol)
Longevity
Guaranteed to provide 15 population doublings when grown in CnT-57 Medium.
Storage / Shelf life
Immediately upon arrival transfer the cryo vial to the liquid nitrogen container, until ready to use.
Thawing
Recommended seeding density after thawing: 4 x 103 cells / cm2. For thawing instructions, please see general cultivation protocol in the resources section of www.cellntec.com
Freezing
Recommended freezing density: 1 x 106 cells / mL. For freezing instructions, please see general cultivation protocol in the resources section of www.cellntec.com
Quality control
Tested negative for Hepatitis B, Hepatitis C and HIV-1. Free of bacteria, fungi and mycoplasma contamination.
Shipping condition
Cells are shipped on dry ice.
Comments
For routine cell cultivation CELLnTEC recommends to work without antibiotics / antimycotics.
Intended use
For research use only. Not for use in therapy or diagnostics.
Last update
2017-05-24