Expression of Desmoglein 2, Desmocollin 3 and Plakophilin 2 in Placenta and Bone Marrow-Derived Mesenchymal Stromal Cells
Authors
Melanie L. Hart & Elisa Rusch & Marvin Kaupp & Kay Nieselt & Wilhelm K. Aicher
Institution
Laboratory for Cell & Tissue Engineering, Department of Orthopeics and Trauma Surgery, Medical Center-Albert-Ludwigs-University of Freiburg
Country
Germany
Year
2017
Journal
Stem Cell Reviews and Reports
Abstract
Many controversial results exist when comparing
mesenchymal stromal cells (MSCs) derived from different
sources. Reasons include not only variables in tissue origin, but
also methods of cell preparation or choice of expansion media
which can strongly influence the expression and hence, function
of the cells. In this short report we aimed to investigate the expression
of the cell anchoring proteins desmoglein 2, desmocollin
3 and plakophilin 2 in early passage placenta-derived MSCs of
fetal (fetal pMSCs) and maternal (maternal pMSCs) origins versus
adult bone marrow-derived MSCs (bmMSCs) that were expanded
and cultured under the same good manufacturing practice
(GMP) conditions. Comprehensive gene expression microarray
analysis profiling indicated differential expression of these genes
in the different MSC-derived types with fetal pMSCs expressing
the highest levels of PKP2, DSC3 and DSG2, followed by maternal
pMSCs, while bmMSCs expressed the lowest levels. A
higher expression of PKP2 and DSC3 genes in fetal pMSCs
was confirmed by qRT-PCR suggesting neonatal increases in
the expression of these desmosomal genes vs. adult MSCs.
Intracellular desmocollin 3 and desmoglein 2 expression was observed
by flow cytometry and cytoplasmic plakophilin 2 by immunofluorescence
in all three MSC sources. These data suggest that fetal pMSCs, maternal pMSCs and bmMSCs may anchor
intermediate filaments to the plasma membrane via desmocollin
3, desmoglein 2 and plakophilin 2.