HPEK_d6

Primary human keratinocytes growing in CnT-PR medium. Day 5 after seeding.

 
 

Double your isolation efficiency from every valuable biopsy! Add Isolation Boosting Supplement (CnT-ISO-50) to epithelial culture medium.

Proliferation Media

CnT-Prime medium is carefully formulated to deliver excellent keratinocyte growth and longevity in a fully defined, animal component free environment.

This is achieved with a combination of Progenitor Cell Targeted (PCT) factors to improve retention of cells in an undifferentiated phenotype, and co-factors to improve growth factor binding and proliferation.

Double your isolation efficiency from every valuable biopsy! Try the CnT-ISO-50 supplement.

Like to try a test sample? Click here for more information.

 

CnT-PR (CnT-Prime) Epithelial Proliferation Medium

  • Precision medium for isolation and expansion of epithelial cells
  • Chemically defined, contains no components of animal or human origin
  • Fully supplemented, one bottle ready-to-go
  • Also available as Higher Certified medium CnT-PR-HC

Features of CnT-PR

 

CnT-07 Epithelial Proliferation Medium

  • Precision medium for isolation and expansion of epithelial cells
  • Chemically defined
  • Also available as Higher Certified medium CnT-07-HC

Features of CnT-07

 

CnT-57 Epithelial Proliferation Medium, Low BPE

  • Medium for isolation and expansion of epithelial cells, in particular if they are difficult to grow
  • Contains a low concentration of BPE

Features of CnT-57

 

HPEK_2D Differentiation

Upregulation of differentiation markers during 8-days following confluence, switch to CnT-PR-D, and addition of calcium. Results courtesy Prof. My Mahoney, Dept. Dermatology, Jefferson University.

Differentiation Media

The keratinocyte differentiation process is a complex and highly-orchestrated series of events.

It is significantly affected by the cell’s environment.

Proliferation media contain a wide range of factors that are directly inhibitory to cell differentiation.

As a result, these media actively retard the differentiation process.

The new CnT-Prime 2D Differentiation medium is specifically designed to encourage complete differentiation of epidermal keratinocytes.

Following calcium switch at confluency, these cells increase expression of a wide range of differentiation markers over a period of one week (see example to the right).

Features of CnT-Prime 2D Diff. (#CnT-PR-D)

Like to try a test sample? Click here for more information.

For recommended differentiation protocols, please visit the Resources for more information.