CELLnTEC
CELLnTEC / Products / CnT-PR-EN

CnT-PR-EN

  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-Prime Endothelial Proliferation Medium

High-efficiency isolation and expansion of primary human endothelial cells in an FBS-free, Xeno-Free, clinically upgradable formulation.

Catalog CnT-PR-EN
Content 500 ml, Frozen medium + serum supplement

HIGHLIGHTS:

  • Longevity of endothelial cells in culture
  • Fully supplemented, one bottle ready-to-go after addition of serum
  • Successor to CnT-ENDO medium

Description

CnT-PR-EN medium is a highly refined formulation for high-efficiency isolation and expansion of primary human endothelial cells. It uses an advanced basal medium supplemented with a range of growth factors and is FBS-free. HANDLING INSTRUCTION: We recommend thawing the medium and serum cautiously. Supplement 10 ml of serum to the 500 ml medium. Upon supplementation, the medium may exhibit a turbid appearance, nevertheless, it remains suitable for utilization without adverse effects. Alternatively, the serum may undergo filtration using a 0.2-micron membrane filter under aseptic conditions before its incorporation into the medium. Likewise, filtration of the serum-supplemented medium can also be performed.

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Specifications

Tissue type
Endothelial cells
Application
Isolation / Expansion
Serum
Low Human
BPE
No
Free of Human & Animal Component
No
Chemically defined
No
Clinically upgradable
Yes
Volume
500 ml
Quality level
Research Grade

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Scientific resources

CnT-ENDO_P5_P9
Figure: Primary HUVEC cells growing in the CnT-PR-EN medium at Passage 3.

Primary human endothelial cells growing in CELLnTEC’s endothelial medium retain enhanced proliferation and extended growth. It uses an advanced basal medium supplemented with a range of growth factors and is FBS-free.

Cells demonstrate the correct localization of VE-Cadherin, a critical regulator of endothelial cell behaviour.

Functionality of the HUVEC cells is also evident in the successful completion of a tube formation assay.

Figure: Primary HUVEC cells demonstrate correct juntional localization of VE-Cadherin under both static and flow (1.4 Pa) conditions. Analysis courtesy of Georgios Stefanopoulos, Laboratory of Thermodynamics in Emerging Technologies, ETH Zurich.
Figure: Tube formation (microvascular-on-a-chip) and in vitro modeling of blood clotting by primary HUVEC cells grown in CELLnTEC’s endothelial medium (upper). Red: live cell staining. Green: Fluorochrom-labelled fibrinogen. Control: cells in EGM-2 medium (lower). Image courtesy of the Schroeder Group, Experimental Haemostasis, University of Bern.

Click to enlarge the image. HUVEC growing in CELLnTEC’s endothelial medium offer increased proliferate rates and longevity in comparison with EGM-2 medium. Image courtesy of the Schroeder Group, Experimental Haemostasis, University of Bern.

For an overview of the performance of HUVEC growing in CELLnTEC’s endothelial medium, please download this A4 Flyer.


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Scientific Literature

Title Authors Year Tissue type
A microfluidic platform integrating functional vascularized organoids-on-chip Clément Quintard, Gustav Jonsson, Camille Laporte, Caroline Bissardon, Amandine Pitaval, Nicolas Werschler, Alexandra Leopoldi, Astrid Hagelkrüys, Pierre Blandin, Jean-Luc Achard, Fabrice Navarro,Yves Fouillet, Josef M. Penninger, Xavier Gidrol 2024 Other
Complement lectin pathway components MBL and MASP-1 promote haemostasis upon vessel injury in a microvascular bleeding model Murielle Golomingi, Jessie Kohler, Lorenz Jenny, Elaissa T. Hardy, Jozsef Dobo, Peter Ga, Gabor Pa, Bence Kiss, Wilbur A. Lam and Verena Schroeder 2022 Other
Real-time monitoring of oxygen levels within thermoplastic Organ-on-Chip devices Anubhav Bussooa, Emily Tubbs, Frederic Revol-Cavalier, Ayman Chmayssem, Manuel Alessio, Marie-Line Cosnier, Nicolas Verplanck 2022 Other
Title Year
CELLnTEC Catalog 2023 Download

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