Effects of serially passaged fibroblasts on dermal and epidermal morphogenesis in human skin equivalents
Authors
David Janson, Marion Rietveld, Rein Willemze, Abdoelwaheb El Ghalbzouri
Institution
Leiden Uni
Country
Netherlands
Year
2013
Journal
Biogerontology
Abstract
Abstract Serial passaging has a profound effect on
primary cells. Since serially passaged cells show signs
of cellular aging, serial passaging is used as an in vitro
model of aging. To relate the effect of in vitro aging
more to in vivo aging, we generated human skin
equivalents (HSEs). We investigated if HSEs generated
with late passage fibroblasts show characteristics
of aged skin when compared with HSEs generated
with early passage fibroblasts. Late passage fibroblasts
had enlarged cell bodies and were more often positive
for myofibroblast marker a-smooth muscle actin,
senescence associated b-galactosidase and p16 compared
with early passage fibroblasts. Skin equivalents
generated with late passage fibroblasts had a thinner
dermis, which could partly be explained by increased
matrix metalloproteinase-1 secretion. In equivalents
generated with late passage fibroblasts epidermal
expression of keratin 6 was increased, and of keratin
10 slightly decreased. However, epidermal proliferation,
epidermal thickness and basement membrane
formation were not affected. In conclusion, compared
with HSEs generated with early passage fibroblasts,
HSEs generated with late passage fibroblasts showed
changes in the dermis, but no or minimal changes in
the basement membrane and the epidermis.
Keywords: Fibroblasts, Human skin equivalents,
Skin aging, Senescence