A Method to Investigate the Epidermal Permeability Barrier In Vitro
Yoshikazu Uchida, Anna Celli
University of California, San Francisco
Molecular Dermatology; Methods in Molecular Biology
The epidermal permeability barrier serves as a multifunctional partition to protect its host from the external environment. Most epidermal permeability barrier studies have been conducted using in vivo human and experimental animals, although some studies have used in vitro cultured cells. There currently is an increased demand for these cultured models, thus avoiding the use of laboratory animals. Here, we first summarize required features that need to be recaptured in cultured keratinocytes for an epidermal permeability barrier study and second, we describe a method for culturing these cells. We also introduce methods to analyze the epidermal permeability barrier function using cultured keratinocytes.
3D epidermal models
Human keratinocytes are isolated from newborn human foreskins