Involvement of nuclear factor of activated T cells in granulocyte–macrophage colony-stimulating factor production in canine keratinocytes stimulated with a cysteine protease
Authors
Tsuyoshi Kimura, Machiko Sekido, Aki Iio, Naoki Chimura, Sanae Shibata, Harumi Kamishina, Hiroaki Kamishina and Sadatoshi Maeda
Institution
Gifu University, Japan
Country
Japan
Year
2013
Journal
Vet Dermatol
Abstract
Background – A previous study demonstrated that the cysteine protease of Dermatophagoides farinae induced
production of granulocyte–macrophage colony-stimulating factor (GM-CSF) in a canine epidermal keratinocyte
progenitor cell line (CPEK); however, the molecular mechanism has not been elucidated.
Hypothesis/Objectives – Given that the transcription of GM-CSF mRNA in human lymphocytes is mainly regulated
by the nuclear factor of activated T cells (NFAT), it is hypothesized that NFAT also contributes to GM-CSF
production in canine keratinocytes stimulated with a cysteine protease.
Methods – Nuclear translocation of NFAT was evaluated in CPEK cells in the absence or presence of the cysteine
protease papain. We also investigated whether blockade of NFAT could inhibit GM-CSF production.
Results – Papain-induced nuclear translocation of NFAT, producing GM-CSF, was partly inhibited by ciclosporin.
Conclusions and clinical importance – The results suggest that GM-CSF production mediated by the cysteine
protease is regulated not only by NFAT but also by unknown signalling pathways in canine keratinocytes.