Liposomal inhibition of acrolein-induced injury in rat cultured urothelial cells
Authors
J. Nirmal, A. S. Wolf-Johnston, M. B. Chancellor, P. Tyagi, M. Anthony, J. Kaufman, L. A. Birder
Institution
Oakland Uni
Country
United States
Year
2014
Journal
Int Urol Nephrol
Abstract
Purpose To study the protection offered by empty liposomes
(LPs) alone against acrolein-induced changes in
urothelial cell viability and explored uptake of LPs by
primary (rat) urothelial cells.
Methods Acrolein was used as a means to induce cellular
damage and reduce urothelial cellular viability. The effect
of acrolein or liposomal treatment on cellular proliferation
was studied using 5-bromo-20-deoxy-uridine assay. Cytokine
release was measured after urothelial cells were
exposed to acrolein. Temperature-dependent uptake study
was carried out for fluorescent-labeled LPs using confocal
microscopy.
Results Liposome pretreatment protected against acrolein-
induced decrease in urothelial cell proliferation. LPs
also significantly affected the acrolein-induced cytokine
(interferon-gamma) release offering protection to the urothelial
cells against acrolein damage. We also observed a
temperature-dependent urothelial uptake of fluorescentlabeled
LPs occurred at 37 C (but not at 4 C).
Conclusions Empty LPs alone provide a therapeutic
efficacy against acrolein-induced changes in urothelial cell
viability and may be a promising local therapy for bladder
diseases. Hence, our preliminary evidence provides support
for liposome-therapy for urothelial protection and possible
repair.
Keywords Urothelium Urinary bladder Liposome
Injury Barrier function