The effects of human keratinocyte coculture on human adipose-derived stem cells
Authors
Bommie F Seo, Ki J Kim, Min K Kim & Jong W Rhie
Institution
Department of Plastic and Reconstructive Surgery, College of Medicine, The Catholic University of Korea, Seoul, South Korea
Country
South Korea
Year
2014
Journal
International Wound Journal
Abstract
The potential for adipose-derived stem cells to differentiate into keratinocyte-like cells
has recently been receiving attention, stemming from the hypothesis that a bioengineered
skin may be manufactured from these readily available mesenchymal stem cells.
This study was conducted to evaluate the influence of human keratinocyte non-contact
coculture on hADSCs. Human epidermal keratinocytes and hADSCs obtained by
lipoaspiration were cultured in keratinogenic growth media, which were divided into the
following groups: human adipose-derived stem cell (hADSC) monoculture, non-contact
coculture of hADSCs and human keratinocytes and keratinocyte monoculture. Cell proliferation
was assessed, and keratogenicity was analysed through immunocytochemistry
and polymerase chain reaction of early, intermediate and late keratogenicmarkers. hADSCs
cocultured with keratinocytes displayed enhanced proliferation compared with the
monoculture group. After a 7-day coculture period, immunohistochemistry and polymerase
chain reaction findings revealed the presence of specific keratinocyte markers
in the coculture group. This study demonstrates that hADSCs cocultured with keratinocytes
have the capacity to transdifferentiate into keratinocyte lineage cells, and
suggests that adipose tissue may be a source of keratinocytes that may further be used
in structuring the bioengineered skin.