Class-leading proliferation of primary human endothelial cells over extended periods, all in a serum-free, clinically ready formulation.
| Catalog | CnT-PR-EN-HC |
|---|---|
| Content | 500 ml, Frozen medium w/o serum |
CnT-PR-EN-HC medium is a highly refined medium for high efficiency isolation and expansion of primary human endothelial cells. The basal medium is also supplemented with a range of Progenitor Cell Targeted (PCT) growth factors to extend longevity and co-factors to improve growth factor binding to membrane-bound receptors. It does not contain phenol-red or antibiotics/antimycotics. CnT-PR-EN-HC medium is ideally suited for use during product development, protocol validation, and characterization of the cell-based product. HANDLING INSTRUCTION: For optimized performance of CnT-PR-EN-HC medium, we recommend sourcing and supplementing 1 % serum suiting your clinical application.
Primary human endothelial cells growing in CELLnTEC’s endothelial medium retain enhanced proliferation and extended growth. It uses an advanced basal medium supplemented with a range of growth factors and is FBS-free. We recommend use of 1 % serum for optimal performance.
Cells demonstrate the correct localization of VE-Cadherin, a critical regulator of endothelial cell behaviour.
Functionality of the HUVEC cells is also evident in the successful completion of a tube formation assay.
Click to enlarge image. HUVEC growing in CELLnTEC’s endothelial medium offer increased proliferate rates and longevity in comparison with EGM-2 medium. Image courtesy of the Schroeder Group, Experimental Haemostasis, University of Bern.
For an overview of the performance of HUVEC growing in CELLnTEC’s endothelial medium, please download this A4 Flyer.
| Title | Authors | Year | Tissue type |
|---|---|---|---|
| A microfluidic platform integrating functional vascularized organoids-on-chip | Clément Quintard, Gustav Jonsson, Camille Laporte, Caroline Bissardon, Amandine Pitaval, Nicolas Werschler, Alexandra Leopoldi, Astrid Hagelkrüys, Pierre Blandin, Jean-Luc Achard, Fabrice Navarro,Yves Fouillet, Josef M. Penninger, Xavier Gidrol | 2024 | Other |
| Complement lectin pathway components MBL and MASP-1 promote haemostasis upon vessel injury in a microvascular bleeding model | Murielle Golomingi, Jessie Kohler, Lorenz Jenny, Elaissa T. Hardy, Jozsef Dobo, Peter Ga, Gabor Pa, Bence Kiss, Wilbur A. Lam and Verena Schroeder | 2022 | Other |
| Real-time monitoring of oxygen levels within thermoplastic Organ-on-Chip devices | Anubhav Bussooa, Emily Tubbs, Frederic Revol-Cavalier, Ayman Chmayssem, Manuel Alessio, Marie-Line Cosnier, Nicolas Verplanck | 2022 | Other |
| Title | Year | |
|---|---|---|
| CELLnTEC Catalog | 2023 | Download |