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CELLnTEC / Products / CnT-PR-EN-HC

CnT-PR-EN-HC

  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-Prime Endothelial Proliferation Medium w/o Serum, Higher Certified

Class-leading proliferation of primary human endothelial cells over extended periods, all in a serum-free, clinically ready formulation.

Catalog CnT-PR-EN-HC
Content 500 ml, Frozen medium w/o serum

HIGHLIGHTS:

  • Longevity of endothelial cells in culture
  • Extended QC, documentation, support, and process control for clinical researchers
  • Serum-free formulation enables the end user to supplement with serum validated to deliver the desired cell behavior and known to meet the necessary regulatory requirements
  • Successor to CnT-ENDO-HC medium

Description

CnT-PR-EN-HC medium is a highly refined medium for high efficiency isolation and expansion of primary human endothelial cells. The basal medium is also supplemented with a range of Progenitor Cell Targeted (PCT) growth factors to extend longevity and co-factors to improve growth factor binding to membrane-bound receptors. It does not contain phenol-red or antibiotics/antimycotics. CnT-PR-EN-HC medium is ideally suited for use during product development, protocol validation, and characterization of the cell-based product. HANDLING INSTRUCTION: For optimized performance of  CnT-PR-EN-HC medium, we recommend sourcing and supplementing 1 % serum suiting your clinical application.

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Specifications

Tissue type
Endothelial cells
Application
Isolation / Expansion
Serum
Supplementation by User
BPE
No
Free of Human & Animal Component
Yes
Chemically defined
Yes
Clinically upgradable
Yes
Volume
500 ml
Component(s)
Frozen Bottle & Ready-to-use after adding serum
Quality level
Higher Certified

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Scientific resources

CnT-ENDO_P5_P9
Figure: Primary HUVEC cells growing in CELLnTEC’s endothelial medium at Passage 5 and Passage 9.

Primary human endothelial cells growing in CELLnTEC’s endothelial medium retain enhanced proliferation and extended growth. It uses an advanced basal medium supplemented with a range of growth factors and is FBS-free. We recommend use of 1 % serum for optimal performance.

Cells demonstrate the correct localization of VE-Cadherin, a critical regulator of endothelial cell behaviour.

Functionality of the HUVEC cells is also evident in the successful completion of a tube formation assay.

Figure: Primary HUVEC cells demonstrate correct juntional localization of VE-Cadherin under both static and flow (1.4 Pa) conditions. Analysis courtesy of Georgios Stefanopoulos, Laboratory of Thermodynamics in Emerging Technologies, ETH Zurich.
Figure: Tube formation (microvascular-on-a-chip) and in vitro modeling of blood clotting by primary HUVEC cells grown in CELLnTEC’s endothelial medium (upper). Red: live cell staining. Green: Fluorochrom-labelled fibrinogen. Control: cells in EGM-2 medium (lower). Image courtesy of the Schroeder Group, Experimental Haemostasis, University of Bern.

Click to enlarge image. HUVEC growing in CELLnTEC’s endothelial medium offer increased proliferate rates and longevity in comparison with EGM-2 medium. Image courtesy of the Schroeder Group, Experimental Haemostasis, University of Bern.

For an overview of the performance of HUVEC growing in CELLnTEC’s endothelial medium, please download this A4 Flyer.


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Scientific Literature

Title Authors Year Tissue type
A microfluidic platform integrating functional vascularized organoids-on-chip Clément Quintard, Gustav Jonsson, Camille Laporte, Caroline Bissardon, Amandine Pitaval, Nicolas Werschler, Alexandra Leopoldi, Astrid Hagelkrüys, Pierre Blandin, Jean-Luc Achard, Fabrice Navarro,Yves Fouillet, Josef M. Penninger, Xavier Gidrol 2024 Other
Complement lectin pathway components MBL and MASP-1 promote haemostasis upon vessel injury in a microvascular bleeding model Murielle Golomingi, Jessie Kohler, Lorenz Jenny, Elaissa T. Hardy, Jozsef Dobo, Peter Ga, Gabor Pa, Bence Kiss, Wilbur A. Lam and Verena Schroeder 2022 Other
Real-time monitoring of oxygen levels within thermoplastic Organ-on-Chip devices Anubhav Bussooa, Emily Tubbs, Frederic Revol-Cavalier, Ayman Chmayssem, Manuel Alessio, Marie-Line Cosnier, Nicolas Verplanck 2022 Other
Title Year
CELLnTEC Catalog 2023 Download

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