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CnT-AG2

  • Isolation
  • Expansion
  • Differentiation
  • Homeostasis
  • Cryopreservation
  • Co-culture
  • Aging
  • Staining

CnT-AG2 VitroAge Keratinocyte Aging Medium

Specifically designed to age epidermal keratinocytes over several passages, without the need for artificial pro-aging treatments such as peroxide. The cells maintain normal morphology, generate several well-documented in vivo signs of aging including reduced lifespan and disrupted metabolism.

Catalog CnT-AG2
Content 500 ml, Frozen medium

HIGHLIGHTS:

  • Avoids artificial and acute pro-aging treamtents such as peroxide, which are potentially damaging
  • Chemically defined
  • Fully supplemented, one bottle ready-to-use

Description

VitroAge medium (CnT-AG2) is specifically designed to age epidermal keratinocytes over several passages, without the need for artificial pro-aging treatments such as perioxide.

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Specifications

Tissue type
Epithelial cells
Species
Human
Application
Aging
Serum
No
BPE
No
Free of Human & Animal Component
No
Chemically defined
Yes
Clinically upgradable
Yes
Volume
500 ml
Component(s)
Frozen Bottle & Ready-to-use
Quality level
Research Grade

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Scientific resources

VitroAge_CumPD_AG2

Loss of cell function with aging also seen as a decrease in proliferation.

Traditional culture media have evolved over 50 years to provide strong proliferation for extended periods. They contain many protective and stimulatory factors and nutrients, that exert a powerful anti-aging effect.

CnT-AG2 lacks these protective elements. Keratinocytes age naturally in this medium over a period of several weeks, without the need for acute doses of oxidizers or other synthetic pro-aging chemicals.

The Vitroage epidermal model maintains normal cell morphology, but generates several well documented in vivo signs of aging including reduced lifespan and disrupted metabolism. For an overview of key ageing-related literature, please click here.

It is now well documented that the aged epidermis is characterized by a reduced number and function of transient-amplifying progenitor cells (Charruyer et al, JID 2009). In particular, these progenitors were found to cycle more slowly and fewer times in aged tissue. A similar reduction in cell cycle kinetics is also observed in VitroAge during 4 weeks of culture (see Fig at left).

Keratinocytes aged in VitroAge medium demonstrate a range of other age-related changes. Please click here to view a poster with additional details.

CnT-AG2 is a fully defined formulation designed to age keratinocytes over several weeks without external chemical stimuli or oxidisers. It does not contain antibiotics / antimycotics.

The VitroAge medium (CnT-AG2) is specifically designed to age epidermal keratinocytes over several passages, without the need for artificial pro-aging treatments such as perioxide.

Isolation, thawing, seeding, and passaging protocols are particularly important for optimal cell growth. Please visit our Protocols Page for our recommendations.
 
AVAILABILITY UPDATE:
The specialty VitroAge medium is available as a custom, made-to-order product. Minimum quantities and a lead time of approx 10 weeks apply. Please enquire via support@cellntec.com for more information.

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Scientific Literature

Title Authors Year Tissue type
Transit-amplifying cell frequency and cell cycle kinetics are altered in aged epidermis Charruyer A, Barland CO, Yue L, Wessendorf HB, Lu Y, Lawrence HJ, Mancianti ML, Ghadially R. 2009 Epidermal
Title Year
CELLnTEC Catalog 2020 Download

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